文献类型：期刊文献

英文题名：Seed Transcriptomics Analysis in Camellia oleifera Uncovers Genes Associated with Oil Content and Fatty Acid Composition

作者：Lin, Ping[1] Wang, Kailiang[1] Zhou, Changfu[2] Xie, Yunhai[3] Yao, Xiaohua[1] Yin, Hengfu[1]

第一作者：林萍

通信作者：Yao, XH[1];Yin, HF[1]|[a00051e90d3009f1c7c75]姚小华;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Hunan Acad Agr Sci, Inst Hort Res, Changsha 410125, Hunan, Peoples R China;[3]Forestry Seedling Management Stn Zhejiang Prov, Hangzhou 310020, Zhejiang, Peoples R China

年份：2018

卷号：19

期号：1

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000424407200115)】；

基金：This work was supported by funds from the National Natural Science Foundation of China (No. 31400580) and the Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (No. CAFYBB2014QB037, CAFYBB2016SZ001).

语种：英文

外文关键词：Camellia oleifera; transcriptomics; lipid biosynthesis; fatty acid; seed oil

摘要：Camellia oleifera is a major tree species for producing edible oil. Its seed oil is well known for the high level of oleic acids; however, little is known regarding the molecular mechanism of lipid biosynthesis in C. oleifera. Here, we measured the oil contents and fatty acid (FA) compositions at four developmental stages and investigated the global gene expression profiles through transcriptomics sequencing. We identified differentially-expressed genes (DEGs) among the developmental stages and found that the distribution of numbers of DEGs was associated with the accumulation pattern of seed oil. Gene Ontology (GO) enrichment analysis revealed some critical biological processes related to oil accumulation, including lipid metabolism and phosphatidylcholine metabolism. Furthermore, we investigated the expression patterns of lipid biosynthesis genes. We showed that most of the genes were identified with single or multiple copies, and some had correlated profiles along oil accumulation. We proposed that the higher levels of stearoyl-ACP desaturases (SADs) coupled with lower activities of fatty acid desaturase 2 (FAD2) might be responsive to the boost of oleic acid at the late stage of C. oleifera seeds' development. This work presents a comprehensive transcriptomics study of C. oleifera seeds and uncovers valuable DEGs that are associated with the seed oil accumulation.