文献类型：期刊文献

中文题名：提高根癌农杆菌介导的香石竹遗传转化效率的研究

英文题名：Establishment of Efficient Agrobacterium tumefaciens-mediated Genetic Transformation System of Carnation(Dianthus caryophyllus L.)

作者：林荣呈[1] 陈龙清[1] 包满珠[1] 孙振元[2]

第一作者：林荣呈

机构：[1]华中农业大学园艺林学学院;[2]中国林业科学研究院花卉中心

年份：2003

卷号：16

期号：2

起止页码：123-128

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助(39970532);国家林业局"948"项目(97 4 06)资助

语种：中文

中文关键词：香石竹;根癌农杆菌;遗传转化;GUS

外文关键词：Dianthus caryophyllus; Agrobacterium tumefaciens; genetic transformation; GUS

分类号：S722.3

摘要：以无菌苗叶片为外植体,在根癌农杆菌介导下建立并优化了香石竹5个品种的遗传转化体系。预培养2d可明显提高转化率;香石竹品种间在转化上存在差异;培养基中添加20μmol的AgNO3抑制不定芽的分化。转化植株在含25mg·L-1卡那霉素的生根培养基上培养,生根率为72 1%,GUS检测结果55%的转化植株呈蓝色,PCR扩增表明阳性率为32 2%,Southern杂交证实外源基因已整合到植物基因组中。
Efficient genetic transformation system of five carnation (Dianthus caryophyllus) cultivars has been established and optimized by using leaf explant. The results show that 2days preculture can increase the transformation frequency obviously. There are differences between carnation cultivars in transformation. 20μmol AgNO3 in selection medium inhibits the adventitious shoot inducing. 721% transformed plants rooted on rooting medium containing 25 mg·L-1 kanamycin and 55% plants show GUS activity according to histochemical assay. PCR screening indicates that 322% of the samples are positive. Southern blot analysis demonstrates that foreign genes are integrated into plant genome.