文献类型：期刊文献

英文题名：Evaluation of Candidate Reference Genes for Quantitative Gene Expression Studies in Tree Peony

作者：Zhou, Lin[] Shi, Qianqian[] Wang, Yan[1] Li, Kui[] Zheng, Baoqiang[] Miao, Kun[]

第一作者：周琳

通信作者：Wang, Y[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China

年份：2016

卷号：141

期号：2

起止页码：99-U127

外文期刊名：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE

收录：;Scopus(收录号：2-s2.0-84962019712);WOS:【SCI-EXPANDED(收录号:WOS:000378141000001)】；

基金：This work was financially supported by National Natural Science Foundation of China (Grant No. 31201654) and the National High Technology Research and Development Program of China (863 Program) (Grant No. 2011AA10020701).

语种：英文

外文关键词：flower color; tissue; development stage; qRT-PCR; geNorm; NormFinder; BestKeeper

摘要：Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) is a sensitive and widely used technique for gene expression analysis that depends on stability of the reference genes used for data normalization. Tree peony (Paeonia suffruticosa), known as one of the most famous traditional ornamental plants in China, is very popular in both domestic and international markets for its showy and colorful flowers. To date, no systematic studies on reference genes have been performed in tree peony with different flower colors. In this study, we evaluated the expression stability of 12 candidate reference genes in different tissues and five flower developmental stages of tree peony with six different colors by three algorithms: geNorm, NormFinder, and BestKeeper. The results showed that protein phosphatase 2A (PP2A), ubiquitin protein ligase (UPL), and ubiquitin (UBQ) were the most stable genes across all samples. Helicase, alpha-tubulin (TUA), and eukaryotic translation initiation factor 5A (EIF5A) also exhibited high expression stability in different tissues, in samples with different colors, and at different flower developmental stages. According to the geNorm analysis, the combination of two most stable reference genes was optimal for normalization in all tested sample sets in this study. To further validate the suitability of the reference genes identified in this study, the expression patterns of two putative homologs of chalcone synthase gene (PsCHS1) and chalcone isomerase gene (PsCHI1) were studied at different developmental stages of white flowers. The results provide information for transcriptional analyses in future studies of gene expression on tree peony flower development and pigmentation.