文献类型：期刊文献

英文题名：De novo Assembly of the Camellia nitidissima Transcriptome Reveals Key Genes of Flower Pigment Biosynthesis

作者：Zhou, Xingwen[1,2] Li, Jiyuan[3] Zhu, Yulin[1,2] Ni, Sui[4] Chen, Jinling[2] Feng, Xiaojuan[2] Zhang, Yunfeng[2] Li, Shuangquan[2] Zhu, Hongguang[1] Wen, Yuanguang[1]

第一作者：Zhou, Xingwen

通信作者：Zhou, XW[1];Wen, YG[1];Zhou, XW[2]|[a00051cc706af4dd001ab]周兴文;[a00057b51efb2107ad3db]温远光;

机构：[1]Guangxi Univ, Coll Forestry, Guangxi Key Lab Forest Ecol & Conservat, Nanning, Peoples R China;[2]Yulin Normal Univ, Coll Biol & Pharm, Yulin, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Peoples R China;[4]Ningbo Univ, Coll Marine Sci, Ningbo, Zhejiang, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000409529300003)】；

基金：This work was supported by the National Natural Science Foundation of China (31360197) and the Foundation of Ecology Center for Post-doctoral Studies, Guangxi University.

语种：英文

外文关键词：RNA-sequencing; transcriptome; metabolic pathway; carotenoids; flavonoids; flower color; Camellia nitidissima

摘要：The golden camellia, Camellia nitidissima Chi., is a well-known ornamental plant that is known as the queen of camellias because of its golden yellow flowers. The principal pigments in the flowers are carotenoids and flavonol glycosides. Understanding the biosynthesis of the golden color and its regulation is important in camellia breeding. To obtain a comprehensive understanding of flower development in C. nitidissima, a number of cDNA libraries were independently constructed during flower development. Using the Illumina Hiseq2500 platform, approximately 71.8 million raw reads (about 10.8 gigabase pairs) were obtained and assembled into 583,194 transcripts and 466, 594 unigenes. A differentially expressed genes (DEGs) and co-expression network was constructed to identify unigenes correlated with flower color. The analysis of DEGs and co-expressed network involved in the carotenoid pathway indicated that the biosynthesis of carotenoids is regulated mainly at the transcript level and that phytoene synthase (PSY), beta-carotene 3-hydroxylase (CrtZ), and capsanthin synthase (CCS1) exert synergistic effects in carotenoid biosynthesis. The analysis of DEGs and co-expressed network involved in the flavonoid pathway indicated that chalcone synthase (CHS), naringenin 3-dioxygenase (F3H), leucoanthocyanidin dioxygenase(ANS), and flavonol synthase (FLS) play critical roles in regulating the formation of flavonols and anthocyanidin. Based on the gene expression analysis of the carotenoid and flavonoid pathways, and determinations of the pigments, we speculate that the high expression of PSY and CrtZ ensures the production of adequate levels of carotenoids, while the expression of CHS, FLS ensures the production of flavonols. The golden yellow color is then the result of the accumulation of carotenoids and flavonol glucosides in the petals. This study of the mechanism of color formation in golden camellia points the way to breeding strategies that exploit gene technology approaches to increase the content of carotenoids and flavonol glucosides and to decrease anthocyanidin synthesis.