文献类型：期刊文献

中文题名：文冠果BZR1基因家族鉴定及功能分析

英文题名：The identification and functional analysis of BZR 1 genes in yellowhorn

作者：许慧慧[1] 班卓[1] 王晨雪[1] 毕泉鑫[1] 刘肖娟[1] 王利兵[1]

第一作者：许慧慧

机构：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,林木遗传育种全国重点实验室,北京100091

年份：2025

卷号：49

期号：2

起止页码：12-22

中文期刊名：南京林业大学学报(自然科学版)

外文期刊名：Journal of Nanjing Forestry University:Natural Sciences Edition

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(32271838)。

语种：中文

中文关键词：文冠果;BZR1转录因子;非生物胁迫;基因过表达;盐胁迫响应;XsBZR1-9

外文关键词：yellowhorn(Xanthoceras sorbifolium);BZR1 transcription factor;abiotic stress tolerance;gene overexpression;salt stress response;XsBZR 1-9

分类号：S722

摘要：【目的】探究文冠果(Xanthoceras sorbifolium)BrassinazoleResistance1(BZR1)基因家族成员的特征及其在非生物胁迫响应中的作用,为文冠果XsBZR1基因功能的研究和抗逆新品种的选育提供理论参考。【方法】利用生物信息学方法鉴定并系统分析文冠果XsBZR1基因家族;构建35S::XsBZR1-eYFP融合蛋白,对XsBZR1进行亚细胞定位分析;通过实时荧光定量PCR技术分析XsBZR1基因在非生物胁迫下的表达模式;构建XsBZR1-9基因的过表达载体并转化到拟南芥(Arabidopsis thaliana)中,观察转基因株系与野生型植株在盐胁迫处理下的生长情况。【结果】(1)在文冠果基因组中共鉴定出9个BZR1基因,分别命名为XsBZR1-1—XsBZR1-9,这些基因不均匀地分布在6条染色体上。(2)系统进化和共线性分析表明,XsBZR1蛋白与双子叶植物的BZR 1转录因子亲缘关系更为密切。(3)XsBZR1基因的启动子区域具有大量的光响应、激素响应元件以及胁迫应答响应元件。(4)亚细胞定位结果和预测结果一致,9个XsBZR1均定位于细胞核。(5)qRT-PCR分析表明,9个XsBZR1基因在不同的非生物胁迫下表现出不同的表达模式。除XsBZR1-7外,其余XsBZR1基因在低温胁迫下3 h时迅速上调表达;盐胁迫下XsBZR1的表达量呈现较大的差异性,XsBZR1-3/4/5/7的表达受到盐胁迫的显著抑制,而XsBZR1-1和XsBZR1-9在盐胁迫处理9 h时表达量提高到约20倍;XsBZR1-3/7/8/9在干旱处理9 h时表达量提高到2倍以上,而其余XsBZR1在干旱处理下的表达水平变化不大;9个XsBZR1均受到ABA的诱导表达,其中XsBZR1-8在ABA处理9 h时表达量提高到35倍。(6)在拟南芥中过表达XsBZR1-9发现,转基因植株在盐胁迫处理后的主根长度显著高于野生型植株。【结论】XsBZR1家族成员参与了文冠果非生物胁迫响应,过表达XsBZR1-9显著提高植物对盐胁迫的耐受性,这为进一步分析XsBZR1基因在文冠果抗逆机制中的作用奠定了基础。
【Objective】This study aimed to systematically characterize the BZR1 transcription factor family in yellowhorn(Xanthoceras sorbifolium)and elucidate its functional roles in abiotic stress response,thereby providing insights for breeding stress-resistant cultivars.【Method】Genome-wide identification of XsBZR 1 genes was performed using bioinformatics tools.Subcellular localization of XsBZR1 proteins was validated via 35S::XsBZR1-eYFP fusion constructs.Expression profiles under abiotic stresses(low temperature,salt,drought)and abscisic acid(ABA)treatment were analyzed by qRT-PCR.Functional validation was conducted by overexpressing XsBZR 1-9 in Arabidopsis thaliana and assessing salt tolerance phenotypes.【Result】Nine XsBZR 1 genes(XsBZR 1-1 to XsBZR 1-9)were identified and distributed unevenly across six chromosomes.Phylogenetic and synteny analyses revealed close evolutionary relationships with dicotyledonous BZR1 orthologs.Promoter regions harbored abundant cis-regulatory elements associated with light responsiveness(48.9%),hormone signaling(35.0%)and stress adaptation(24.6%).The nuclear localization of all XsBZR1 proteins was experimentally confirmed.Differential expression patterns were observed under stress conditions:low temperature(4℃)rapidly induced eight XsBZR1 genes(1.3-to 6.0-fold upregulation at 3 h;P<0.05),except XsBZR 1-7.Salt stress(150 mmol/L NaCl)suppressed XsBZR 1-3/4/5/7 but strongly upregulated XsBZR 1-1(26.1-fold)and XsBZR 1-9(19.6-fold)at 9 h(P<0.001).Drought stress(mass fraction 25%PEG6000)elevated XsBZR 1-3/7/8/9 expression(>1.9-fold at 9 h),while others remained stable.ABA treatment(100μmol/L)universally induced XsBZR 1 genes,with XsBZR 1-8 showing a 35.4-fold increase(P<0.001).Transgenic Arabidopsis overexpressing XsBZR 1-9 exhibited enhanced salt tolerance,with taproot lengths twice that of the wild-type under 100 mmol/L NaCl(P<0.01).【Conclusion】The XsBZR 1 gene family plays pivotal roles in yellowhorn's abiotic stress response,with XsBZR 1-9 demonstrating significant potential for improving salt tolerance.These findings advance the molecular understanding of stress adaptation mechanisms in woody plants and provide targets for precision breeding.