文献类型：期刊文献

英文题名：Identification and Functional Prediction of Poplar Root circRNAs Involved in Treatment With Different Forms of Nitrogen

作者：Zhou, Jing[1] Yang, Ling-Yu[] Jia, Chen-Lin[] Shi, Wen-Guang[] Deng, Shu-Rong[] Luo, Zhi-Bin[]

第一作者：周婧

通信作者：Zhou, J[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture Natl Forestry, Beijing, Peoples R China; Chinese Acad Forestry, Res Inst Forestry, Grassland Adm, Beijing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85134704231);WOS:【SCI-EXPANDED(收录号:WOS:000830186500001)】；

基金：The platform of the facilities from the State Key Laboratory of Tree Genetics and Breeding is acknowledged.

语种：英文

外文关键词：circular RNAs; ceRNA regulatory network; Populus x canescens; roots; different nitrogen forms

摘要：Circular RNAs (circRNAs) are a class of noncoding RNA molecules with ring structures formed by covalent bonds and are commonly present in organisms, playing an important regulatory role in plant growth and development. However, the mechanism of circRNAs in poplar root responses to different forms of nitrogen (N) is still unclear. In this study, high-throughput sequencing was used to identify and predict the function of circRNAs in the roots of poplar exposed to three N forms [1 mM NO3- (T1), 0.5 mM NH4NO3 (T2, control) and 1 mM NH4+ (T3)]. A total of 2,193 circRNAs were identified, and 37, 24 and 45 differentially expressed circRNAs (DECs) were screened in the T1-T2, T3-T2 and T1-T3 comparisons, respectively. In addition, 30 DECs could act as miRNA sponges, and several of them could bind miRNA family members that play key roles in response to different N forms, indicating their important functions in response to N and plant growth and development. Furthermore, we generated a competing endogenous RNA (ceRNA) regulatory network in poplar roots treated with three N forms. DECs could participate in responses to N in poplar roots through the ceRNA regulatory network, which mainly included N metabolism, amino acid metabolism and synthesis, response to NO3- or NH4+ and remobilization of N. Together, these results provide new insights into the potential role of circRNAs in poplar root responses to different N forms.