文献类型：期刊文献

中文题名：应用nanoLC-MS/MS分析毛白杨次生维管系统的蛋白质表达谱

英文题名：Proteome Profile in Secondary Vascular System of Populus tomentosa by nanoLC-MS/MS

作者：蒋淑磊[1,2] 陈加飞[1,3] 赵树堂[1,2] 卢孟柱[1,2]

第一作者：蒋淑磊

机构：[1]林木遗传育种国家重点实验室;[2]中国林业科学研究院林业研究所;[3]中国林业科学研究院林业新技术研究所

年份：2013

卷号：49

期号：5

起止页码：43-53

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金重点项目(31030018)

语种：中文

中文关键词：液相色谱-串联质谱;蛋白质表达谱;次生维管系统;毛白杨

外文关键词：LC-MS/MS; proteome profile; secondary vascular system; Populus tomentosa

分类号：S718.46

摘要：基于1DE的nanoLC-MS/MS方法分析毛白杨次生维管系统的蛋白质组,成功鉴定:14个蛋白质参与细胞分裂,7个转录因子,22个与细胞骨架相关蛋白质,31个与细胞信号转导相关蛋白质,14个与细胞壁相关蛋白质。显示这些蛋白质相应基因参与形成层活动的调控,为进一步了解木材形成的分子机制奠定基础。
As the uppermost carbon pool and important renewable source, wood has dual functions in both responding to the global climate change and meeting the resource demands of human society, thereby it is essential to understand the molecular mechanism of wood formation. In this study, a 1DE-based nanoLC-MS/MS method was introduced to analyze the proteome in the secondary vascular system of Populus tomentosa, and the identified proteins included：14 proteins associated cell division; 7 proteins related to transcription factors; 22 proteins associated with cytoskeleton; 31 proteins associated with signal transduction;and 14 proteins associated with cell wall formation. The corresponding genes of these proteins participate in regulating cambial activity. The accomplishment of this proteome analysis laid a foundation for further understanding the molecular mechanism of wood formation.