文献类型：期刊文献

中文题名：应用单拷贝核基因标记研究我国欧洲山杨自然居群的遗传多样性

英文题名：A Study on Genetic Diversity of Populus tremula in China by Single-copy Nuclear Markers

作者：杜淑辉[1,2] 王兆山[1] 保尔江·阿布都哈米提[3] 邢世岩[2] 张建国[1]

第一作者：杜淑辉

机构：[1]国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]山东农业大学林学院,山东泰安271000;[3]新疆阿勒泰地区林业科学研究所,新疆阿勒泰836500

年份：2016

卷号：29

期号：1

起止页码：48-52

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金(31470665);北京市优秀博士学位论文指导教师科技项目;江苏省高等教育联合创新计划

语种：中文

中文关键词：新疆;欧洲山杨;单拷贝核基因标记;遗传多样性;遗传分化

外文关键词：Xinjiang; Populus tremula; sing-copy nuclear markers; genetic diversity; genetic differentiation

分类号：S718.46

摘要：[目的]对分布于我国新疆地区欧洲山杨自然居群的遗传多样性与遗传分化进行研究。[方法]对4个欧洲山杨自然居群共72个个体的6个单拷贝核基因标记(sing-copy nuclear markers)进行扩增与测序,并计算相应的遗传多样性和遗传分化参数。[结果]表明:比对后6个基因的长度在459 bp到747 bp之间,平均多态核苷酸位点个数为14,遗传多样性指数π和θw分别达到了0.004 8和0.004 4,基因多样性指数为0.73,以上结果表明欧洲山杨表现出较高的遗传多样性水平。Tajima中性检验结果均不显著,表明所用6个单拷贝核基因均符合中性进化假设。AMOVA分析表明89.72%的遗传变异存在于居群内,居群间的平均遗传分化指数Fst为0.10,居群间平均基因流(Nm)为3.235。[结论]高度异交,高碱基突变率及超长距离的基因流机制能够很好的解释我国新疆地区欧洲山杨表现出的较高的遗传多样性水平与较低水平的遗传分化。
[Objective]This paper aims at investigating the genetic diversity and genetic differentiation of Populus tremula distributed in Xinjiang,China. [Method]Six single-copy nuclear gene markers of 72 P. tremula individuals from 4 populations in Xinjiang were amplified and sequenced. The parameters of genetic diversity and genetic differentiation were calculated. [Result]After aligning,the length of the six genes ranged between 459 bp and 747 bp and the average number of polymorphism sites was 14. Diversity parameters,π and θw,reached 0. 004 8 and0. 004 4,respectively. The mean value of gene diversity was 0. 73. These indicated high level of genetic diversity of P. tremula. The results of Neutral tests using Tajima＇s D were not significant,which indicated all the six genes did not violate from neutrality. The results of AMOVA analysis showed that 89. 72% of the total variance existed within populations. The average genetic differentiation parameter Fstand gene flow among populations reached 0. 10 and3. 235,respectively. [Conclusion]High level of outcrossing rate and nucleotide mutation rate as well as long distance dispersal seed contributed to the high level of genetic diversity and low genetic differentiation in P. tremula inXinjiang,China.