文献类型：期刊文献

中文题名：卡特兰ACC氧化酶cDNA片断的克隆与序列分析

英文题名：Cloning and sequencing analysis of cDNA encoding ACC oxidase from Cattleya

作者：郑宝疆[1] 郑宝强[1] 王雁[1] 彭镇华[1] 李晓华[2] 齐靖[3] 李莉[3]

第一作者：郑宝疆

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]国际竹藤网络中心国家林业局竹藤科学与技术重点实验室,北京100102;[3]河北农业大学园艺学院,河北保定071001

年份：2008

卷号：31

期号：6

起止页码：43-46

中文期刊名：河北农业大学学报

外文期刊名：Journal of Agricultural University of Hebei

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD_E2011_2012】；

基金：国家林业局948项目(2006-4-C07,2005-4-37)

语种：中文

中文关键词：卡特兰;ACC氧化酶;RT—PCR;序列分析

外文关键词：Cattleya ; ACC oxidase; RT - PCR ; sequence analysis

分类号：S682.31

摘要：以卡特兰(Cattleya)花朵为试材,首先提取总RNA,并根据其它兰花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase,ACO)基因保守序列设计1对特异性引物,然后通过RT-PCR法,克隆得到一条卡特兰ACC氧化酶的cDNA片断,该片断长度为967 bp,共编码321个氨基酸残基。序列分析结果显示该克隆片断与已发表的其它兰花的ACC氧化酶基因序列同源性很高,均在85%以上,尤其与其近亲C.bicolor、C.inter-media、Laelia anceps的同源性均在95%以上。
Total RNA was extracted from flowers of Cattleya. According to the conserved acid sequence for ACC oxidase in other orchids, a pair of Oligo nucleotide primers were designed. Using RT- PCR method, a cDNA fragment about 967 base pair which encoded 321 predicted amino acid residues were amplified. The result of Blast showed the sequence presented a very high match with the ACO genes from the other orchids, and the homologue was higher than 85 %, especially the relatives of C. bicolor, C. intermedia and Laelia anceps whose homologue was higher than 95%.