文献类型：期刊文献

英文题名：WRKY14-DPB Module Enhances Drought Tolerance by Activating the Expression of UGT84B1 Involved in Hydrolyzable Tannin Biosynthesis

作者：Gao, Jing[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Zhao, Yunxiao[1,2] Wang, Yangdong[1,2]

通信作者：Zhao, YX[1];Wang, YD[1];Zhao, YX[2];Wang, YD[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China

年份：2025

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-105010583680);WOS:【SCI-EXPANDED(收录号:WOS:001529411600001)】；

基金：We are very grateful to Dr. Yan Pu for providing BiFC and VIGS vectors. This study was supported by National Key Research and Development Program of China (2022YFD2200603); Forestry and Grassland Science and Technology Innovation Young Top Talents; Zhejiang Province Key Research and Development Program (2024C02009).

语种：英文

外文关键词：DPB; drought; hydrolyzable tannin; UGT84B; WRKY

摘要：Drought stress severely limits the growth and development of trees. Tannins, which serve as vital secondary metabolites in plant roots, help mitigate drought stress. The Lauraceae family, which holds major economic and ecological value, faces substantial developmental challenges due to its sensitivity to drought conditions. Despite this, research on the regulatory mechanisms governing tannin-specific accumulation under drought stress remains limited. In this study, we aim to explore how WRKY14 interacts with DPB to regulate the metabolism of hydrolyzable tannin (HT) via the key enzyme UGT84B1, thereby enhancing drought tolerance in Litsea cubeba, a main species within the Lauraceae family. The WRKY-DPB-UGT84B1 module was specifically expressed in roots in response to drought stress. LcUGT84B1 was found to generate 1-O-Galloyl-beta-d-glucose in vitro and in overexpressing L. cubeba. Moreover, molecular biology and transformation experiments demonstrated that LcWRKY14 and LcDPB formed a complex that directly bound to the LcUGT84B1 promoter, activating its expression and thereby facilitating HT synthesis. Co-overexpression of LcWRKY14 and LcDPB significantly enhanced drought tolerance by increasing HT accumulation. These findings provide new insights into the regulatory mechanisms of the WRKY-DPB-UGT84B1 module in promoting drought tolerance and offer a potential breeding strategy for developing drought-resistant varieties.