文献类型：期刊文献

英文题名：Insights into PeERF168 gene in slash pine terpene biosynthesis: Integrating high-throughput phenotyping, GWAS, and transgenic studies

作者：Ding, Xianyin[1,2] Diao, Shu[1,2] Zhang, Yini[1] Luan, Qifu[1,2] Li, Yanjie[1,2] Jiang, Jingmin[1,2] Wu, Harry X.[3]

第一作者：Ding, Xianyin

通信作者：Luan, QF[1];Li, YJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Natl Forestry & Grassland Adm, Hangzhou 311400, Peoples R China;[3]Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, SE-90183 Umea, Sweden

年份：2025

卷号：300

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20250517780331);Scopus(收录号：2-s2.0-85216224660);WOS:【SCI-EXPANDED(收录号:WOS:001415017700001)】；

基金：We are grateful to our colleagues at the Research Institute of Subtropical Forestry, Chinese Academy of Forestry (RISF, CAF) for assistance with sampling. This research was funded by the Science and Technology Innovation 2030 Program, Ministry of Science and Technology, People's Republic of China (2023ZD0405805) and the National Key Research and Development Program of Ministry of Science and Technology of the People's Republic of China (2022YFD2200023-2).

语种：英文

外文关键词：Terpene biosynthesis; High-throughput phenotyping; GWAS; eQTL mapping; PeERF168; Slash pine

摘要：Resin biosynthesis in conifer is a complex process, controlled by multiple quantitative trait loci (QTLs). Quantifying resin components is traditionally expensive and labor-intensive. In this study, we employed near infrared (NIR) spectroscopy to quantify resin components in Slash pine using 240 genotypes. A partial least squares regression model was applied to identify the characteristic bands responsed to variations in Alpha and Beta pinene levels. Genome-wide association study (GWAS) identified 35 significant SNPs involved in terpenoid precursor biosynthesis, transport, modification, and abiotic stress resistance. eQTL mapping co-localized four candidate genes: PeCHITINASE (c166891.graph_c0), PeGLYCOSYLTRANSFERASE (c160167.graph_c0), PeASIL2 (c324347.graph_c0), and PeERF168 (c311225.graph_c0). Mutations in two SNPs increased the expression of PeASIL2 and PeERF168, leading to higher levels of Alpha and Beta pinene. Further heterologous transformation experiments confirmed that the PeERF168 gene regulates the concentration of both monoterpenes and sesquiterpenes. These findings provide valuable insights into the molecular mechanisms of resin biosynthesis, facilitating cost-effective gene discovery through high-throughput resin component detection and genomics integration, with substantial potential to enhance molecular breeding and improve resin yield and quality.