文献类型：期刊文献

英文题名：Abscisic acid enhances lead translocation from the roots to the leaves and alleviates its toxicity in Populus x canescens

作者：Shi, Wen-Guang[1] Liu, Wenzhe[1] Yu, Wenjian[1] Zhang, Yuhong[1] Ding, Shen[2] Li, Hong[3] Mrak, Tanja[4] Kraigher, Hojka[4] Luo, Zhi-Bin[1]

第一作者：石文广

通信作者：Luo, ZB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Silviculture,State Key Lab Tree Genet & B, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[3]Chinese Acad Forestry, Postgrad Sch, Beijing 100091, Peoples R China;[4]Slovenian Forestry Inst, Vecna Pot 2, Ljubljana, Slovenia

年份：2019

卷号：362

起止页码：275-285

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000449127500031)】；

基金：This study was jointly supported by the Fundamental Research Funds for the Central Non-profit Research Institutions of CAF (grant nos. CAFYBB2018SY005 and CAFYBB2018ZY001-2) and the National Natural Science Foundation of China (Grant Nos. 31470618 and 31670609). Dr Duncan Jackson from the United Kingdom is sincerely thanked for correcting the English language in this manuscript.

语种：英文

外文关键词：Detoxification; Heavy metal; Phytohormone; Populus; Transcriptional regulation

摘要：To shed light on physiological mechanisms underlying abscisic-acid (ABA)-mediated lead (Pb) uptake, trans location and detoxification, we exposed Populus x canescens saplings to either 0 or 3 mM Pb2+ in combination with either 0 or 10 mu M exogenous ABA. Pb was taken up by the roots and accumulated mainly in the cortex. A fraction of the Pb in the roots was translocated to the leaves, thereby resulting in decreased photosynthesis and biomass. Pb accumulation caused a burst of reactive oxygen species (ROS), with higher concentrations of total thiols, glutathione, and ascorbate in the roots and/or leaves. Exogenous ABA stimulated Pb uptake, decreased Pb deposition in the cortex, and enhanced Pb vascular loading in the roots. Exogenous ABA alleviated the Pb-induced reductions in photosynthesis and root biomass, and decreased Pb-triggered ROS overproduction in the roots and/or leaves. Correspondingly, exogenous ABA stimulated the mRNA levels of a few genes involved in Pb uptake, transport, and detoxification, including NRAMPI.4, ABCG40, FRD3.1, PCS1.1, and ABCC1.1. These results suggest that exogenous ABA enhances Pb uptake and translocation, and alleviates Pb toxicity in poplars through the ABA-induced movement of Pb from the root cortex to the vascular stele, and transcriptionally regulated key genes involved in Pb tolerance.