文献类型：期刊文献

中文题名：滇牡丹花瓣着色过程中类胡萝卜素成分变化和相关基因表达分析

英文题名：Changes of Carotenoid Components and Expression of the Related Genes During Petal Coloring of Paeonia delavayi

作者：邹红竹[1] 周琳[1] 韩璐璐[1] 吕纪杭[1] 王雁[1]

第一作者：邹红竹

机构：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091

年份：2021

卷号：48

期号：10

起止页码：1934-1944

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划项目(2018YFD1000405);国家自然科学基金项目(31972456)。

语种：中文

中文关键词：滇牡丹;花色;类胡萝卜素;基因表达

外文关键词：Paeonia delavayi;flower color;carotenoid;gene expression

分类号：S685.11

摘要：滇牡丹(Paeonia delavayi)中的黄色花类群是十分重要的育种资源。为厘清其花瓣着色过程中类胡萝卜素成分及含量变化,以花发育过程中4个时期的花瓣为材料进行类胡萝卜素定性及定量分析,并对类胡萝卜素生物合成途径中的关键结构基因PdPSY、PdZDS、PdLCYB、PdCHY、PdNXS、PdCRISTO、PdCHYB和降解途径中关键结构基因PdCCD的转录水平进行实时荧光定量PCR分析。首次在滇牡丹花瓣中鉴定出14种类胡萝卜素类物质,分别是α–胡萝卜素、花药黄质、番茄红素、玉米黄质、紫黄质、γ–胡萝卜素、新黄质、β–胡萝卜素、叶黄素、β–隐黄质、β–阿朴胡萝卜素醛、八氢番茄红素、ε–胡萝卜素和α–隐黄质,其中含量最高的为叶黄素和八氢番茄红素。随着花发育,八氢番茄红素、番茄红素和γ–胡萝卜素的积累量不断上升;α–胡萝卜素、β–胡萝卜素、α–隐黄质、叶黄素、紫黄质、新黄质的积累量则不断下降。荧光定量结果显示PdPSY、PdCRISTO、PdZDS、PdLCYB和PdCCD表达量呈上升趋势,PdCHYE、PdCHYB和PdNXS表达量呈下降趋势,其中PdCHYB和PdNXS在透色期开始表达量明显降低;PdCHYE持续降低,该趋势与色素含量变化基本一致。
The yellow flower group in Paeonia delavayi is an important breeding resource.In order to clarify the changes of carotenoid components and content during petal coloring,carotenoids in the petals from four stages of flower development were analyzed qualitatively and quantitatively,and the key structural genes of PdPSY,PdZDS,PdLCYB,PdCHY,PdNXS,PdCRISTO,PdCHYB in carotenoid biosynthesis pathway and of PdCCD in degradation pathway were analyzed by Real-time fluorescence quantitative PCR.In this study,14 carotenoids were identified in the petals of Paeonia delavayi for the first time,and they wereα-carotene,antheraxanthin,lycopene,zeaxanthin,violaxanthin,γ-carotene,neoxanthin,β-carotene,lutein,β-cryptoxanthin,apocarotenal,(E/Z)-phytoene,ε-carotene andα-cryptoxanthin.Among them,lutein and(E/Z)-phytoene had the highest content.With the development of flowers,the accumulation of(E/Z)-phytoene,lycopene andγ-carotene increased continuously,while the accumulation ofα-carotene,β-carotene,α-cryptoxanthin,lutein,violaxanthin and neoxanthin decreased continuously.The results of Real-time quantitative PCR showed that the expression of PdPSY,PdCRISTO,PdZDS,PdLCYB and PdCCD presented an upward trend,the expression of PdCHYE,PdCHYB and PdNXS showed a downward trend,among which the expression of PdCHYB and PdNXS decreased significantly at slightly pigmented soft bud stage,and the expression of PdCHYE decreased gradually during petal coloring,which was basically consistent with the change of pigment content.