文献类型：期刊文献

英文题名：A comparison of next-generation sequencing with clone sequencing in the diet analysis of Asian great bustard

作者：Gong, Minghao[1,2] Ning, Yu[1,2] Han, Morigen[3] Zhao, Chenyang[4] Tian, Jingxing[4] Li, Linwei[5] Xiao, Hongye[1,2] Liu, Gang[1,2]

第一作者：龚明昊;Gong, Minghao

通信作者：Liu, G[1];Liu, G[2]

机构：[1]Chinese Acad Forestry, Inst Wetland Res, Beijing 100091, Peoples R China;[2]Beijing Key Lab Wetland Serv & Restorat, Beijing 100091, Peoples R China;[3]Tumuji Natl Nat Reserve, Tumuji 137600, Inner Mongolia, Peoples R China;[4]Cangzhou Normal Coll, Dept Biol, Cangzhou 061001, Peoples R China;[5]Hebei Normal Univ, Shijiazhuang 050024, Hebei, Peoples R China

年份：2019

卷号：11

期号：1

起止页码：15-17

外文期刊名：CONSERVATION GENETICS RESOURCES

收录：;Scopus(收录号：2-s2.0-85062266382);WOS:【SCI-EXPANDED(收录号:WOS:000460097500004)】；

基金：This work was financed by the Fundamental Research Funds of CAF (Grant No. CAFINT2015K05), and the National Natural Science Foundation (No. 31500303).

语种：英文

外文关键词：Diet analysis; Non-invasive fecal DNA; Next-generation sequencing; Clone sequencing; Asian great bustard

摘要：Comparative studies of next-generation sequencing (NGS) and clone sequencing in diet analysis have not been conducted. This study compares the performance of NGS and clone sequencing to analyze the diet of Asian great bustard (Otis tarda dybowskii). Identification of known taxa using NGS method was significantly greater than by clone method (t=8.12, df=14, p<0.01), which has highlighted the higher sensitivity of the NGS approach. The proportion of occurrence for NGS method and clone method was significantly correlated (r=0.63, p=0.01), suggesting the prey taxa present at low frequency could not be identified by clone method. The cost and time of NGS method was also low compared with the clone method. We recommend the NGS opens up for new possibilities in the diet study, and has wide applicability to other species whose faeces can be collected easily and non-invasively in the field.