文献类型：期刊文献

中文题名：反硝化芽孢杆菌的筛选鉴定及反硝化特性

英文题名：Screening and identification of denitrifying bacillus strain and its denitrification characteristics

作者：郑喜春[1] 郭晓军[1] 姚娜[2] 曹晓璐[2] 李潞滨[2] 朱宝成[1]

第一作者：郑喜春

机构：[1]河北农业大学生命科学学院;[2]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室

年份：2012

卷号：31

期号：6

起止页码：1447-1452

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家水体污染控制与治理科技重大专项(2009ZX07212-004-3-5);中央级公益性科研院所基本科研业务费专项资金(RIF2010-16)资助

语种：中文

中文关键词：反硝化细菌;脱氮率;巨大芽孢杆菌

外文关键词：denitrifying bacteria; nitrogen removal rate; Bacillus megaterium.

分类号：Q939.9

摘要：采用加热富集及硝酸盐还原产气试验和反硝化活力定量分析,从乌梁素海的底泥中筛选出了一株具有高效反硝化性能的芽孢杆菌,命名为TGR30。通过形态观察、生理生化试验及16SrDNA序列同源性分析,将其鉴定为巨大芽孢杆菌(Bacillus megaterium)。通过单因素试验明确了碳源、碳氮比、盐浓度、pH以及溶解氧与反硝化特性的关系。结果表明,该菌在盐度为6%或pH12时都具有较高的反硝化活性;以可溶性淀粉为唯一碳源、C/N为14、pH8.0、DO为4mg·L-1时脱氮效果最佳,脱氮率为92%。
By the methods of heating accumulation, nitrate-reduction gas production, and quantitative analysis of denitrification, a bacillus strain with high-efficient denitrifying capability named as TGR30 was isolated from the sediment of Wuliangsuhai Lake. The isolate was identified as Bacillus megaterium, based on its morphological feature, biochemical characteristics, and 16S rDNA sequence analysis. Single factor experiment was conducted to investigate the influences of medium carbon, C/N, salinity, pH, and dissolved oxygen on the denitrifying characteristics of the strain. When the medium salinity was 6% or the pH value was 12, the strain still had high nitrogen removal capability, and the optimal conditions for the nitrogen removal were the medium C/N ratio 14, pH value 8.0, and dissolved oxygen concentration 4 mgL-1 when soluble starch was used as the sole carbon source.