文献类型：期刊文献

英文题名：Halophyte Nitraria billardieri CIPK25 mitigates salinity-induced cell damage by alleviating H2O2 accumulation

作者：Lu, Lu[1] Wu, Xinru[1] Wang, Pengkai[1] Zhu, Liming[1] Liu, Yuxin[1] Tang, Yao[1] Hao, Zhaodong[1] Lu, Ye[1] Zhang, Jingbo[2] Shi, Jisen[1] Cheng, Tielong[3] Chen, Jinhui[1]

第一作者：Lu, Lu

通信作者：Chen, JH[1]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forest Genet & Biotechnol, Minist Educ China, Nanjing, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou, Peoples R China;[3]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85136507830);WOS:【SCI-EXPANDED(收录号:WOS:000891367000001)】；

基金：Funding This research was supported by the Nature Science Foundation of China (32071784), the Youth Foundation of the Natural Science Foundation of Jiangsu Province (Grant No. BK20210614), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：halophyte; Nitraria billardieri; CIPK25; salt tolerance; H2O2

摘要：The plant-specific module of calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) play a crucial role in plant adaptation to different biotic and abiotic stresses in various plant species. Despite the importance of the CBL-CIPK module in regulating plant salt tolerance, few halophyte CIPK orthologs have been studied. We identified NbCIPK25 in the halophyte Nitraria billardieri as a salt-responsive gene that may improve salt tolerance in glycophytes. Sequence analyses indicated that NbCIPK25 is a typical CIPK family member with a conserved NAF motif, which contains the amino acids: asparagine, alanine, and phenylalanine. NbCIPK25 overexpression in salt-stressed transgenic Arabidopsis seedlings resulted in enhanced tolerance to salinity, a higher survival rate, longer newly grown roots, more root meristem cells, and less damaged root cells in comparison to wild-type (WT) plants. H2O2 accumulation and malondialdehyde (MDA) content were both deceased in NbCIPK25-transgenic plants under salt treatment. Furthermore, their proline content, an important factor for scavenging reactive oxygen species, accumulated at a significantly higher level. In concordance, the transcription of genes related to proline accumulation was positively regulated in transgenic plants under salt condition. Finally, we observed a stronger auxin response in salt-treated transgenic roots. These results provide evidence for NbCIPK25 improving salt tolerance by mediating scavenging of reactive oxygen species, thereby protecting cells from oxidation and maintaining plant development under salt stress. These findings suggest the potential application of salt-responsive NbCIPK25 for cultivating glycophytes with a higher salt tolerance through genetic engineering.