文献类型：期刊文献

英文题名：Analysis of culm elongation in photoheterotrophic status of Dendrocalamus sinicus by comparative proteomics

作者：Cui, Kai[1] Wang, Haiying[1] He, Caiyun[2] Sun, Di[3] Zhang, Junpei[2] Liao, Shengxi[1] Cui, Yongzhong[1] Li, Li[1]

通信作者：Liao, Shengxi|[a000570213870d4dc4083]廖声熙;

机构：[1] Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming, 650224, China; [2] Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [3] Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, 77843, United States

年份：2017

卷号：31

期号：2

起止页码：687-704

外文期刊名：Trees - Structure and Function

收录：EI(收录号：20164803064820);Scopus(收录号：2-s2.0-84996956135)

语种：英文

外文关键词：Biochemistry - Physiology - Enzyme activity - Metabolism - Tissue - Molecular biology - Photosynthesis - Starch - Transcription

摘要：Key message: The differential expression of 36 proteins plays a central role in culm elongation during photoheterotrophic status, which was involved in complicated biochemical and molecular processes, including replication, transcription, translation, energy metabolism, central intermediary metabolism, and woody tissue photosynthesis. Abstract: Dendrocalamus sinicus is known as the largest bamboo species in the world, characterized by huge woody culms with rapid growth rate. During culm elongation, leaves are not yet shaped and culms are covered by nontransparent overlapping sheaths, forming a photoheterotrophic status in which culms rapidly complete their own growth. To elucidate this phenomenon, proteome analysis as well as enzyme activity were performed. In terms of the enzyme activity, we found significant differences among internodes for adenosine diphosphoglucose pyrophosphorylase, starch synthase, and starch branching enzyme, implying existence of a robust response of starch synthesis throughout culm elongation. At the protein level, 2-DE combined with MALDI–TOF/TOF MS analysis showed 36 differentially accumulated proteins among internodes. Of which, the largest group is the cluster for energy metabolism (27.78%), followed by central intermediary metabolism (22.22%), which suggests that carbohydrate metabolism dominates the process of culm elongation. Especially, four proteins related to the glycolysis pathway were differentially expressed. In addition, a protochlorophyllide reductase associated with chlorophyllide synthesis was down-regulated from basal to top internodes. This implies that culms belong to photosynthetic tissue and woody tissue photosynthesis is involved in the culm elongation. In addition, malate dehydrogenase related to TCA cycle was up-regulated from tender to mature tissues, which indicates that it plays a vital role in energy generation. An inconsistency between the transcript and protein level was found, suggesting that post-transcriptional events are involved in the culm elongation. These results are helpful to disclose the multiple mechanism of culm elongation of D. sinicus. ? 2016, Springer-Verlag Berlin Heidelberg.