文献类型：期刊文献

中文题名：云南野生黄牡丹谷胱甘肽转移酶(GST)基因的分离及表达分析

英文题名：Isolation and Expression Analysis of GST Gene Encoding Glutathione S-Transferase of Paeonia delavayi var. lutea Wild Population in Yunnan

作者：史倩倩[1] 周琳[1] 王雁[1]

第一作者：史倩倩

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2014

卷号：50

期号：12

起止页码：63-72

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"农村领域国家科技计划课题(SQ2010AA1000687008);国家自然科学基金项目(31201654)

语种：中文

中文关键词：黄牡丹;谷胱甘肽转移酶;花色素;基因克隆;基因表达

外文关键词：Paeonia delavayi var. lutea; glutathione S-transferase; anthocyanin; gene clone; gene expression

分类号：S718.46

摘要：从已构建的云南野生黄牡丹花瓣转录组数据库中得到10个与花色素转运相关的谷胱甘肽转移酶(GST)蛋白同源性高的Unigene序列,分别命名为Pl GST1-10。利用RT-PCR和RACE技术对Pl GST1-10最大阅读框(ORF)序列扩增并进行氨基酸序列比较和系统进化树分析,结果显示:Pl GST5可能与花色素转运相关,包含1个675 bp的开放阅读框,编码1个224个氨基酸的蛋白,含有2个内含子和3个外显子,属于phi型GST;其氨基酸序列与葡萄Vv GST4、矮牵牛Ph An9、仙客来Ckm GST3、拟南芥At TT19、瓜叶菊Sc GST3和香石竹Dc GSTF2等花色素转运GST具有较高的相似性。Pl GST5在不同花色的花发育时期及盛开期的不同组织的qRT-PCR分析表明Pl GST5在花色素积累较多的组织中高丰度表达,在黄牡丹和紫牡丹的盛开期表达量最高,而在牡丹品种‘赵粉’和‘玉板白’的花发育早期表达量最高。推测Pl GST5参与黄牡丹花色素转运。
In this study, 10 unigene sequences that share high homology with glutathione S-transferase（GST） protein involved in plant anthocyanin transfering were obtained from previously-constructed tree peony （Paeonia delavayi var. lutea） petal transcriptome database and named PlGST1 -10. Sequences of Open Reading Frame （ORF） in PlGST1 -10 were amplified with designed specific primers using RT-PCR and RACE techniques, and the amino acid sequences were compared and the phylogenetic tree was analyzed. Results showed that PIGST5 was considered to be an alternative gene which is related to anthocyanin transferring, contained a 675 bp ORF encoding 224 amino acid residues with 2 introns and 3 excons and belonged to phi type GST. The predicted protein sequence of PlGST5 also shared high similarity with other GSTs that are related to anthocyanin transferring, such as VvGST4 （Vitis vinifera）, PhAn9 （Petunia hybrida）, CkmGST3 （Cyclamen persicum ）, AtTT19 （Arabidopsis thaliana ）, ScGST3 （Senecio cruentus ） and DcGSTF2 （Dianthus caryophyllus）. The expression patterns of PIGST5 in different tissues and petals with different flower colors during flower development were investigated using qRT-PCR. The results indicated that PlGST5 had a higher expression level in the tissues containing more anthocyanin. The expression level was the highest when flowers fully opened in P. delavayi var. lutea and P. delavayi, while the expression reached the highest abundance at early developmental stage of P. suffruticosa ＇ Zhao Fen＇ and P. suffruticosa ＇ Yu Banbai＇. In conclusion, we inferred that PlGST5 might associate with the transfer of anthocyanin in P. delavayi var. lutea and this would provide the basis for insight into the molecular mechanisms underlying tree peony yellow flower pigmentation.