文献类型：期刊文献

英文题名：Genome-Wide Identification and Function of Aquaporin Genes During Dormancy and Sprouting Periods of Kernel-Using Apricot (Prunus armeniaca L.)

作者：Li, Shaofeng[1] Wang, Lin[2] Zhang, Yaoxiang[1] Zhu, Gaopu[2] Zhu, Xuchun[2] Xia, Yongxiu[1] Li, Jianbo[1] Gao, Xu[1] Wang, Shaoli[1] Zhang, Jianhui[3] Wuyun, Ta-na[2] Mo, Wenjuan[1]

第一作者：李少锋

通信作者：Wang, SL[1];Wuyun, TN[2];Zhang, JH[3]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Natl Permanent Sci Res Base Warm Temperate Zone F, Beijing, Peoples R China;[2]Chinese Acad Forestry, Nontimber Forestry Res & Dev Ctr, State Key Lab Tree Genet & Breeding, Zhengzhou, Peoples R China;[3]Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85117168200);WOS:【SCI-EXPANDED(收录号:WOS:000717108000001)】；

基金：Funding This work was supported by the Fundamental Research Funds of CAF (CAFYBB2018MA003), the National Natural Science Foundation of China (31770705 and 31400570), and the National Key R&D Program of China (2019YFD1001200).

语种：英文

外文关键词：cold resistance; functional analysis; genome-wide analysis; aquaporin gene; kernel-using apricot (P; armeniaca L; )

摘要：Aquaporins (AQPs) are essential channel proteins that play a major role in plant growth and development, regulate plant water homeostasis, and transport uncharged solutes across biological membranes. In this study, 33 AQP genes were systematically identified from the kernel-using apricot (Prunus armeniaca L.) genome and divided into five subfamilies based on phylogenetic analyses. A total of 14 collinear blocks containing AQP genes between P. armeniaca and Arabidopsis thaliana were identified by synteny analysis, and 30 collinear blocks were identified between P. armeniaca and P. persica. Gene structure and conserved functional motif analyses indicated that the PaAQPs exhibit a conserved exon-intron pattern and that conserved motifs are present within members of each subfamily. Physiological mechanism prediction based on the aromatic/arginine selectivity filter, Froger's positions, and three-dimensional (3D) protein model construction revealed marked differences in substrate specificity between the members of the five subfamilies of PaAQPs. Promoter analysis of the PaAQP genes for conserved regulatory elements suggested a greater abundance of cis-elements involved in light, hormone, and stress responses, which may reflect the differences in expression patterns of PaAQPs and their various functions associated with plant development and abiotic stress responses. Gene expression patterns of PaAQPs showed that PaPIP1-3, PaPIP2-1, and PaTIP1-1 were highly expressed in flower buds during the dormancy and sprouting stages of P. armeniaca. A PaAQP coexpression network showed that PaAQPs were coexpressed with 14 cold resistance genes and with 16 cold stress-associated genes. The expression pattern of 70% of the PaAQPs coexpressed with cold stress resistance genes was consistent with the four periods [Physiological dormancy (PD), ecological dormancy (ED), sprouting period (SP), and germination stage (GS)] of flower buds of P. armeniaca. Detection of the transient expression of GFP-tagged PaPIP1-1, PaPIP2-3, PaSIP1-3, PaXIP1-2, PaNIP6-1, and PaTIP1-1 revealed that the fusion proteins localized to the plasma membrane. Predictions of an A. thaliana ortholog-based protein-protein interaction network indicated that PaAQP proteins had complex relationships with the cold tolerance pathway, PaNIP6-1 could interact with WRKY6, PaTIP1-1 could interact with TSPO, and PaPIP2-1 could interact with ATHATPLC1G. Interestingly, overexpression of PaPIP1-3 and PaTIP1-1 increased the cold tolerance of and protein accumulation in yeast. Compared with wild-type plants, PaPIP1-3- and PaTIP1-1-overexpressing (OE) Arabidopsis plants exhibited greater tolerance to cold stress, as evidenced by better growth and greater antioxidative enzyme activities. Overall, our study provides insights into the interaction networks, expression patterns, and functional analysis of PaAQP genes in P. armeniaca L. and contributes to the further functional characterization of PaAQPs.