文献类型：期刊文献

中文题名：基于表型性状和SSR标记的樟子松核心种质构建

英文题名：Construction of a core collection of Pinus sylvestris var.mongolica based on phenotypic traits and SSR markers

作者：尹明宇[1,2,3,4] 吴波[1,2,3] 乌云塔娜[4] 庞营军[1,2,3] 张学利[5]

第一作者：尹明宇

机构：[1]中国林业科学研究院生态保护与修复研究所,北京100091;[2]中国林业科学研究院荒漠化研究所,北京100091;[3]中国林业科学研究院荒漠生态系统与全球变化国家林业和草原局重点实验室,北京100091;[4]中国林业科学研究院经济林研究所,林木遗传育种国家重点实验室,河南郑州450003;[5]辽宁省沙地治理与利用研究所,辽宁章古台科尔沁沙地生态系统国家定位观测研究站,辽宁阜新123000

年份：2025

卷号：45

期号：6

起止页码：182-190

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划项目(2023YFF1305304);科技兴蒙行动重点专项(KJXM-EEDS-2020006)。

语种：中文

中文关键词：樟子松;核心种质;表型;SSR标记;DNA指纹图谱

外文关键词：Pinus sylvestris var.mongolica;core collection;phenotype;SSR marker;DNA fingerprinting

分类号：S722;S718.46

摘要：【目的】基于表型性状和SSR标记构建樟子松核心种质,为樟子松种质资源有效管理和利用奠定基础。【方法】以科尔沁沙地31年生517份樟子松种质为材料,探究其表型性状(胸径、树高、枝下高、冠幅、单株材积、冠形、干形和分枝)变异规律,应用12对SSR引物分析其遗传多样性。结合表型和SSR数据,依据等位基因数目最大化策略与表型极值和稀有等位基因优先原则构建核心种质。通过表型变异和遗传多样性相关参数差异检验以及主坐标分析评价核心种质的代表性和有效性。【结果】樟子松种质资源具有丰富的表型变异和遗传多样性,各性状变异系数为13.01%~48.21%,表型多样性指数(H′)为0.913~2.064;12个SSR位点共检测到97个等位基因(Na),各位点有效等位基因数(Ne)、Shannon-Wiener多样性指数(I)、观测杂合度(Ho)、期望杂合度(He)和多态性信息含量(PIC)的平均值分别为3.324、1.189、0.511、0.556和0.512。以8.90%的取样比例得到46份核心种质,其均值差异百分率(MD)、方差差异百分率(VD)、极差符合率(CR)和变异系数变化率(VR)分别为1.42%、46.33%、100.00%、135.34%;核心种质的H′、Ne、I、He和PIC均显著高于原有种质且在原有种质中呈均匀分布。应用11个SSR位点构建了46份核心种质的唯一性DNA指纹图谱。【结论】构建的46份樟子松核心种质,能够有效代表樟子松种质资源的表型变异和遗传多样性。
【Objective】The core collection of Pinus sylvestris var.mongolica on sandy lands was constructed on the basis of phenotypic traits and SSR markers,so as to provide foundations for effective management and utilization of its germplasm resources.【Method】Phenotypic traits were investigated in 517 germplasms from 31-year-old plantations of P.sylvestris var.mongolica in Horqin Sandy Land,including the diameter at breast height,tree height,height to live crown base,crown width,individual tree volume,stem form,crown shape and branchiness,and their genetic diversity were assessed using 12 pairs of SSR primers.The core collection were constructed based on these data of phenotypic traits and SSR m,arkers using allele number maximization strategy in conjunction with the principles of extreme phenotype and rare allele prioritization.The representativeness and validity of the core collection were further evaluated through difference tests for parameters of phenotypic variation and genetic diversity as well as analysis of principal coordinate plots.【Result】There are rich phenotypic variation and genetic diversity in collected germplasm resources of P.sylvestris var.mongolica,with the coefficients of variation and the phenotypic diversity index(H′)for each trait ranging from 13.01%to 48.21%and from 0.913 to 2.064,respectively.A total of 97 alleles(Na)are detected at 12 SSR loci,and the mean values of the number of effective alleles(Ne),Shannon-Wiener diversity index(I),observed heterozygosity(Ho),expected heterozygosity(He),and the polymorphic information content(PIC)are 3.324,1.189,0.511,0.556 and 0.512,respectively.A total of 46 core collections are obtained at a sampling rate of 8.90%,and their mean difference percentage(MD),variance difference percentage(VD),coincidence rate of range(CR),and variable rate for the coefficient of variation(VR)are 1.42%,46.33%,100.00%,and 135.34%,respectively.The core collection exhibit significantly higher coefficients of H′,Ne,I,He,and PIC than the initial collection,and the core collections are uniformly distributed throughout the initial collection.The unique DNA fingerprints are also constructed for 46 core germplasm at 11 loci.【Conclusion】The 46 core germplasm collection can effectively represent the phenotypic variation and genetic diversity of the initial collection.