文献类型：期刊文献

英文题名：Isolation and characterisation of a cold-induced DREB gene from Cymbidium insigne

作者：Li, Lu-Bin[1] Zhao, Hua[2] Liu, Lei[2] He, Cong-Fen[2] Bai, Rui[2] Wang, Tao[1] Yao, Na[1]

第一作者：李潞滨

通信作者：Li, LB[1]

机构：[1]Chinese Acad Forestry, State Forestry Adm Res, Key Lab Tree Breeding & Cultivat, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Beijing Technol & Business Univ, Beijing Key Lab Plant Resources Res & Dev, Beijing 100037, Peoples R China

年份：2011

卷号：86

期号：1

起止页码：43-49

外文期刊名：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287947700008)】；

基金：We thank Professor Ma Youzhi for kindly providing the wild-type and mutant DRE-yeast strains, the YepGAP yeast expression vector, the 163GFP vector, the pBI121 vector, and Agrobacterium tumefaciens strain C58c1. We also thank Dr. Xu Zhaoshi and his students for their advice and help. This work was supported by grants from the Forest Scientific and Technical Supporting Programme (No. 2006BAD01A18), the Advanced Agricultural Science and Technology Introduction of "948 Program" (No. 2006-4-C08), and the Major Science and Technology Programme of Hainan Province, P.R. China. (No. 080102).

语种：英文

摘要：Dehydration-responsive element DNA binding (DREB) proteins mediate responses to several environmental stress in plants. A novel CiDREB1 gene of 1,265 bp was isolated from Cymbidium insigne by RACE. The deduced amino acid sequence had a predicted molecular mass of 34.43 kDa and a pI of 5.20. A BLAST search revealed that the encoded protein could be classified as a member of the AP2/EREBP family. Expression analysis showed that CiDREB1 gene transcripts accumulated rapidly under cold stress and peaked by 6 h. Yeast one-hybrid analysis indicated that CiDREB1 could interact with its DNA binding domain to activate expression of the dual reporter genes histidine (His3) and galactosidase (LacZ). CiDREB1 localised to the nucleus, although it lacked the typical nuclear localisation signal (NLS). We inserted the CiDREB1 gene into the binary transformation vector, pBI121 which was then transferred into tobacco plants using Agrobacterium. Forty-seven transgenic tobacco lines were identified by kanamycin and carbenicillin selection. Integration of the foreign gene into the tobacco genome was confirmed by PCR analysis. Furthermore, seven transgenic tobacco plants displayed enhanced tolerance to cold stress applied to whole plants. Thus, CiDREB1 appears to function as a trans-acting factor and may be used to improve tolerance to cold stress in plants.