文献类型：期刊文献

中文题名：β-葡萄糖苷酶和醇酰基转移酶对中国水仙花挥发性成分的影响

英文题名：Effects of β-glucosidase and alcohol acyltransferase on volatiles of Narcissus tazetta var. chinensis

作者：彭爱铭[1] 彭镇华[2] 高健[1]

第一作者：彭爱铭

机构：[1]国际竹藤网络中心;[2]中国林业科学研究院花卉中心

年份：2011

卷号：38

期号：1

起止页码：101-105

中文期刊名：安徽农业大学学报

外文期刊名：Journal of Anhui Agricultural University

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：948引进国际先进林业科学技术重大项目"花卉良种繁育与精确栽培技术创新"(2006-4-C07)资助

语种：中文

中文关键词：β-葡萄糖苷酶;醇酰基转移酶;中国水仙;挥发性成分

外文关键词：β-glucosidase; alcohol acyltransferase; Narcissus tazetta var. chinensis,＂ volatiles

分类号：S682.21

摘要：采用顶空固相微萃取(HS-SPME)-气相色谱/质谱(GC/MS)联用技术分析了中国水仙花主要挥发性成分芳樟醇、乙酸苯甲酯及乙酸苯乙酯的含量,并测定了醇酰基转移酶、β-葡萄糖苷酶的活性。伴随着花的发育,芳樟醇含量在花蕾期含量最高,然后逐渐下降;乙酸苯甲酯、乙酸苯乙酯含量呈先升高后降低的趋势,盛开期含量最高;β-葡萄糖苷酶活性在盛花期最高;醇酰基转移酶活性从花蕾期到盛花期变化不大,盛花期后明显下降。结果表明乙酸苯甲酯、乙酸苯乙酯的含量与β-葡萄糖苷酶、醇酰基转移酶有一定的相关性。
The volatile components of flower of Narcissus tazetta var. chinensis, mainly including linalool, phenylmethyl acetate and phenylethyl acetate were analyzed by the headspace-solid phase micro-extraction coupled to gas chromatography/mass（GC/MS）, meanwhile, the activities ofβ-glucosidase and alcohol acyltransferase were measured. With the development of the flower, the content of linalool decreased gradually, and the maximum concentration was found at the flower bud stage. Phenylmethyl phenylmethyl acetate and phenylethyl acetate increased firstly and decreased in succession, and their concentrations were highest at the full opening stage. The activity of β-glucosidase was the strongest at the full opening stage. The activity of alcohol acyltransferase did not vary, but obviously decreased after the full opening stage. The outcome of the experiment indicated that the concentrations of phenylmethyl acetate and phenylethyl acetate were relative to the activities of β-glucosidase and alcohol acvltransferase.