文献类型：期刊文献

英文题名：Cloning and expression analysis of an E-class MADS-box gene from Populus deltoides

作者：Zhang, Bingyu[1] Zhang, Xianghua[1] Li, Hong[1] Zhou, Xiangming[2] Su, Xiaohua[1]

第一作者：张冰玉

通信作者：Zhang, BY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[2]Tianjin Res Ctr Agr Biotechnol, Tianjin 300192, Peoples R China

年份：2009

卷号：8

期号：19

起止页码：4789-4796

外文期刊名：AFRICAN JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000273274900012)】；

基金：We thank the Eleventh Five National Key Technology R&D program (2006BAD01A15), the National Basic Research Program (2009CB119107) and the National Natural Science Foundation of China (30200225) for financial support.

语种：英文

外文关键词：Floral development; expression; MADS-box; E-class

摘要：An E-class MADS-box gene, PdMADS2, was isolated from Populus deltoides Bartr. ex Marsh male floral buds by RT-PCR. As shown by relative-quantitative real-time polymerase chain reaction analysis, the expression of PdMADS2 was high in apical and floral buds, intermediate in immature xylem and roots and low in mature leaves of adult male P. deltoides. In developing male floral buds, PdMADS2 expression was high and remained constant from July to September, increased significantly on February 6 and quickly fell to a very low level with the maturation of the flowers. In male inflorescences, PdMADS2 expression was abundant in the perianth cups and inflorescence peduncles; it was not detected in mature pollen. In female inflorescences, PdMADS2 was highly expressed in both ovaries and inflorescence peduncles. Our results suggest that PdMADS2 plays an important role in the development of inflorescence meristems and flower organs in poplar.