文献类型：期刊文献

中文题名：茶尺蠖核型多角体病毒(EoNPV)的PCR检测方法及其生物活性研究

英文题名：Application of PCR Method for Detecting of the EoNPV in Ectropis oblique and Its Biological Activity

作者：张永安[1] 仲国立[1] 侯玉霞[1] 贡玉梅[2] 曲良建[3] 王玉珠[3]

第一作者：张永安

机构：[1]中国农业大学理学院;[2]国家林业局科技发展中心;[3]中国林业科学研究院森林生态环境与保护研究所

年份：2008

卷号：21

期号：4

起止页码：451-455

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家科技支撑计划资助(2006BAD08A1202);国家林业局重点项目(2005-09)

语种：中文

中文关键词：EoNPV;茶尺蠖;PCR检测;生物活性

外文关键词：EoNPV ; Ectropis obliqua ; PCR detection ; biological activity

分类号：Q966

摘要：根据茶尺蠖核型多角体病毒(EoNPV)多角体蛋白基因建立了PCR检测方法,在茶尺蠖子代卵和蛹内检测到EoNPV存在,灵敏度达到1 fg,此方法可用于田间施用EoNPV后茶尺蠖种群带毒检测。PCR方法由于操作简单、检测灵敏度高、特异性好、重复性好等优点,值得应用。利用EoNPV感染茶尺蠖2龄幼虫,幼虫对EoNPV的敏感性很高,测得LC50为2.2×104PIB.mL-l,EoNPV感染浓度越高,则幼虫死亡越快,幼虫死亡时间与感染浓度呈正相关。
The polymerase chain reaction （PCR） method for detecting Ectropis obliqua multiple nuclear polyhedrosis virus was established with self-designed primers according to the sequence of polyhedron gene of EoNPV, and EoNPV could be detected from the offspring, the data showed that the method would allow the detection of about 1 fg, this method could be used to identify EoNPV in field practices. The method was simple with good reproducibility and selectivity, and high sensitivity, so deserves to be popularized. An infectivity test of EoNPV against the second instar larvae of Ectropis oblique was carried out in our laboratory, and LC50 of EoNPV was determined as 2.2×10^4 PIB·mL^-1, the larva was very sensitive to EoNPV, and it had a higher death rate with higher EoNPV concentration, and the death time oresented oositive correlation with the concentration of EoNPV.