文献类型：期刊文献

中文题名：白僵菌和绿僵菌蛋白对松墨天牛致病性的研究

英文题名：Pathogenicity of entomogenous fungi proteins to Monochamus alternatus

作者：李建庆[1] 张永安[2] 梅增霞[1] 石东里[1]

第一作者：李建庆

机构：[1]滨州学院生命科学系;[2]中国林业科学研究院森林生态环境与保护研究所

年份：2006

卷号：26

期号：2

起止页码：165-168

中文期刊名：福建林学院学报

外文期刊名：Journal of Fujian College of Forestry

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD_E2011_2012】；

基金：国家重点基础研究发展规划"973"项目(G19990160)

语种：中文

中文关键词：松墨天牛;致病蛋白;球孢白僵菌;金龟子绿僵菌

外文关键词：Monochamus alternatus ; pathogenic protein ; Beauveria bassiana ; Metarhizium anisopliae

分类号：Q968.1

摘要：从4株白僵菌和1株绿僵菌中提取了10种全蛋白提取液,以林业害虫松墨天牛幼虫为试虫,筛选出菌株Bb36的全蛋白提取液Bb36W-Z和Bb36N-Z,再从中分离出12种单一蛋白提取液,生测发现虫生真菌蛋白Bb36W-D对松墨天牛幼虫具有较强的注射毒性,经其处理后的试虫酯酶谱发生明显变化,致使松墨天牛幼虫内部组织发生病变.
Ten total protein extractions were purified from four strains of Beauveria bassiana and one Metarhizium anisopliae. Total protein extraction Bb36W-Z and Bb36N-Z from strain Bb36 were screened by bioassay which objection was Monochamus alternatus larvae, a serious forest pest. Twelve proteins were injected M. a/ternatus larvae after isolated from Bb36W-Z and Bb36N-Z. It was discovered that protein Bb36W-D was higher pathogenicity than others, and caused M. alternatus larvae pathological changes because isozyme patternsof the treatment was different from the control