文献类型：期刊文献

英文题名：Comprehensive Proteome and Acetylome Analysis of Needle Senescence in Larix gmelinii

作者：Zhang, Xuting[1] Shan, Jinyuan[1] Wang, Jiaxiu[1] Zhang, Yanxia[1] Yang, Feiyun[1,2] Liu, Bin[1] Zhang, Lifeng[3] Li, Guojing[1] Wang, Ruigang[1]

第一作者：Zhang, Xuting

通信作者：Li, GJ[1];Wang, RG[1]

机构：[1]Inner Mongolia Agr Univ, Key Lab Plants Advers Adaptat & Genet Improvement, Hohhot 010018, Peoples R China;[2]Inner Mongolia Agr Univ, Coll Food Sci & Engn, Hohhot 010018, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2024

卷号：25

期号：13

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85198422213);WOS:【SCI-EXPANDED(收录号:WOS:001270480300001)】；

基金：This work was supported by the Science and Technology Innovation 2030-Agricultural Biological Breeding Major Project (2023ZD040580205), IMAU & Inner Mongolia Autonomous Region University Science and Technology Innovation Team (NMGlRT2222 & BR22-13-05).

语种：英文

外文关键词：Larix gmelinii; needle senescence; proteome; acetylome

摘要：Leaf senescence is essential for the growth and development of deciduous trees in the next season. Larix gmelinii, a deciduous coniferous tree, exhibits its most distinctive feature by turning yellow in the autumn and eventually shedding its leaves, resulting in significant changes in its appearance during the fall. Lysine acetylation plays an important role in diverse cellular processes; however, limited knowledge is available regarding acetylations in the needle senescence of L. gmelinii. In this study, the proteomics and acetylated modification omics of two phenotypic leaves, yellow and green (senescent and non-senescent) needles, were analyzed before autumn defoliation. In total, 5022 proteins and 4469 unique acetylation sites in 2414 lysine acylated proteins were identified, and this resulted in the discovery of 1335 differentially expressed proteins (DEPs) and 605 differentially expressed acetylated proteins (DAPs) in yellow versus green needles. There are significant differences between the proteome and acetylome; only 269 proteins were found to be DEP and DAP, of which 136 proteins were consistently expressed in both the DEP and DAP, 91 proteins were upregulated, and 45 proteins were down-regulated. The DEPs participate in the metabolism of starch and sucrose, while the DAPs are involved in glycolysis and the tricarboxylic acid cycle. Among them, DEPs underwent significant changes in glycolysis and citric acid cycling. Most of the enzymes involved in glycolysis and the citrate cycle were acetylated. DAPs were down-regulated in glycolysis and up-regulated in the citrate cycle. In all, the results of this study reveal the important role of lysine acetylation in the senescence of L. gmelinii needles and provide a new perspective for understanding the molecular mechanism of leaf senescence and tree seasonal growth.