文献类型：期刊文献

英文题名：Molluscicidal activity and physiological toxicity of quaternary benzo[c]phenanthridine alkaloids (QBAs) from Macleaya cordata fruits on Oncomelania hupensis

作者：Ke, Wenshan[1,2] Tu, Chang[1,2] Cao, Dezhi[3] Lin, Xiong[1,2] Sun, Qiqiang[4] Zhang, Qian[4]

第一作者：Ke, Wenshan

通信作者：Ke, WS[1];Ke, WS[2]

机构：[1]Hubei Univ, Green Resources Transformat & Collaborat Innovat, Wuhan, Hubei, Peoples R China;[2]Hubei Univ, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Hubei, Peoples R China;[3]Huanghuai Univ, Affiliated Hosp 1, Zhumadian, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China

年份：2019

卷号：13

期号：10

外文期刊名：PLOS NEGLECTED TROPICAL DISEASES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000494982700014)】；

基金：This work was supported by The National Key Technology R&D Program of China on Construction Technology of Eco-economic Schistosomiasis Control Forest (No. 2015BAD07B07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Schistosomiasis is a serious worldwide parasitic disease. One of the best ways to control schistosomiasis is to control the population of Oncomelania hupensis snails. We sought to identify a high-efficiency biogenic molluscicide against Oncomelania with low toxicity, to avoid chemical molluscicide contamination and toxicity in aquatic organisms. We extracted quaternary benzo[c]phenanthridine alkaloids (QBAs) from Macleaya cordata fruits. Molluscicidal activity of the QBAs against Oncomelania was determined using bioassay. Our results showed that the extracted QBAs had a strong molluscicidal effect. In treatment of O. hupensis with QBAs for 48 h and 72 h, the lethal concentration (LC50) was 2.89 mg/L and 1.29 mg/L, respectively. The molluscicidal activity of QBAs was close to that of niclosamide (ethanolamine salt), indicating that QBAs have potential development value as novel biogenic molluscicides. We also analyzed physiological toxicity mechanisms by examining the activity of several important detoxification enzymes. We measured the effect of the extracted QBAs on the activities of glutathione S-transferase (GST), carboxylesterase (CarE), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the liver of O. hupensis. We found that the effects of QBAs on detoxification metabolism in O. hupensis were time and concentration dependent. The activities of GST, CarE, AKP, and ACP in the liver of snails increased significantly in the early stage of treatment (24 h), but decreased sharply in later stages (120 h), compared with these activities in controls. GST, CarE, AKP, and ACP activity in the liver of snails treated with LC50 QBAs for 120 h decreased by 62.3%, 78.1%, 59.2%, and 68.6%, respectively. Our results indicate that these enzymes were seriously inhibited by the extracted QBAs and the detoxification and metabolic functions of the liver gradually weakened, leading to poisoning, which could be the main cause of death in O. hupensis snails.