文献类型：期刊文献

中文题名：小叶杨PsWOX11基因对侧根生长发育的影响

英文题名：Effects of PsWOX11 on Lateral Root Growth and Development of Populus simonii

作者：孟佳慧[1,2] 刘宏超[1,2] 武志[3] 左雯腾[1,2] 王留强[1,2]

第一作者：孟佳慧

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,北京100091;[2]南京林业大学,南方现代林业协同创新中心,江苏南京210037;[3]北京市大兴区林场,北京102699

年份：2024

卷号：37

期号：6

起止页码：54-61

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：生物育种国家科技重大专项(2023ZD0407501)。

语种：中文

中文关键词：小叶杨;PsWOX11;侧根;根生长;根直径

外文关键词：Populus simonii;PsWOX11;lateral root;Root growth;Root diameter

分类号：S718.46

摘要：[目的]分析小叶杨PsWOX11基因对侧根生长发育的影响,为研究木本植物WOX11基因调控侧根生长发育的分子作用机制提供参考。[方法]利用生物信息学方法鉴定小叶杨PsWOX11基因,并对基因、蛋白结构以及保守序列等进行分析。利用qRT-PCR技术分析PsWOX11基因组织特异性表达模式。创制转基因植株35S::PsWOX11(OE)和35S::PsWOX11-SRDX(DR),分析过表达和显性抑制PsWOX11基因对转基因杨树侧根生长发育的影响。[结果]PsWOX11基因编码区核苷酸序列长度为768 bp,编码255个氨基酸残基,在杨树主根和侧根中表达丰度相对较高。创制了7个PsWOX11过表达转基因杨树株系和8个显性抑制转基因杨树株系,对不同基因型杨树进行表型及显微形态观察发现,与非转基因84K杨相比,过表达PsWOX11转基因杨树的侧根长度、直径均显著增加,而显性抑制转基因杨树表型相反。[结论]PsWOX11基因参与调控杨树的侧根的生长发育,促使侧根的长度和直径增加,该研究为进一步揭示WOX11基因参与调控小叶杨根系生长提供了参考依据。
[Objective]The effects of PsWOX11 gene on the growth and development of lateral root in Populus simonii were analyzed to provide reference for exploring the molecular mechanism of root growth and development regulated by WOX11 gene in woody plants.[Method]Bioinformatics methods were used to analyze gene and protein structures,and conserved motifs.The tissue-specific expression patterns were analyzed by qRT-PCR.Overexpression(35S::PsWOX11,OE)and dominant repression(35S::PsWOX11-SRDX,DR)transgenic PsWOX11 poplar plants were generated and used to analyze the effects of PsWOX11 gene on the growth and development of lateral root.[Result]The PsWOX11 gene,with 768 bp in length and encoding a 255 amino acid protein,was cloned from Populus simonii.The expression level of PsWOX11 gene was relatively high in the main roots and lateral roots of poplar.Transgenic poplar lines overexpressing PsWOX11(OE1–OE7)and PsWOX11 dominant repression plants(SRDX1–SRDX8)were generated,and the phenotype and microscopic morphology of different genotypes of poplars showed that the lateral root length and diameter of transgenic poplars overexpressing PsWOX11 were significantly higher than those of non-transgenic 84K poplar plants,while opposite phenotypes were observed for PsWOX11 dominant repression plants.[Conclusion]PsWOX11 gene is involved in regulating the growth and development of lateral roots in poplar,which increases the length and diameter of lateral roots.This study provides a reference for further understanding that WOX11 gene is involved in regulating root growth of poplar.