文献类型：期刊文献

英文题名：Cytogenetic analysis of reciprocal hybrids and their parents between Larix leptolepis and Larix gmelinii: implications for identifying hybrids

作者：Zhang, Shou Gong[1] Yang, Wen Hua[1] Han, Su Ying[2] Han, Bo Tao[3] Li, Mao Xue[4] Qi, Li Wang[1]

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[4]Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

年份：2010

卷号：6

期号：3

起止页码：405-412

外文期刊名：TREE GENETICS & GENOMES

收录：;Scopus(收录号：2-s2.0-77955056911);WOS:【SCI-EXPANDED(收录号:WOS:000275976300005)】；

基金：We are grateful to Guang-Yuan Rao and YanPing Guo for their valuable comments on the manuscript. This work was supported by the National High Technology Research and Development Program of China (Grant Nos. 2006AA100109, 2007AA021403, 2007AA10Z182, 2008AA10Z126, and 2007AA021501), the National Natural Science Foundation of China (Grant Nos. 30571517 and 30830086), and the National Basic Research Program of China (Grant Nos. 2009CB119106 and 2009CB119107).

语种：英文

外文关键词：Forest tree hybrids; In situ hybridization; Larix; Meiosis; Pollen mother cells

摘要：We studied the parental taxa and the interspecific reciprocal hybrids between Larix leptolepis with Larix gmelinii, using classical cytogenetic methods, as well as fluorescence in situ hybridization (FISH) and genomic in situ hybridization. A high frequency (> 90%) of complete bivalent formation was observed in reciprocal hybrids. Less than 10% of pollen mother cells exhibited abnormalities. The most frequent abnormalities were bridges. Multivalent chromosome associations were also observed in both reciprocal hybrids, suggesting that some chromosome interchange events did occur, and introgressions from one to the other species were possible. Intergenomic recombination indicates that genes might be readily introgressed into one species from the other in the genus Larix. Interspecific hybridization may be a potential method for genetic improvement in larch. FISH markers documented that the recombinant genomes of reciprocal hybrids were strictly additive and stable, indicating that FISH also might be a useful tool in Larix breeding.