文献类型：期刊文献

英文题名：Structure and Monomer Ratio of Lignin in C3H and HCT RNAi Transgenic Poplar Saplings

作者：Zhou, Xianwu[1,2] Yang, Sheng[2] Lu, Mengzhu[3] Zhao, Shutang[3] Cai, Liping[4] Zhang, Yaoli[5] Zhao, Rongjun[2] Lv, Jianxiong[2]

第一作者：Zhou, Xianwu

通信作者：Zhao, RJ[1];Lv, JX[1]|[a000555af1057d20aad88]赵荣军;

机构：[1]Jishou Univ, Key Lab Hunan Forest & Chem Ind Engn, 120 Ren Min South Rd, Jishou 416000, Peoples R China;[2]Chinese Acad Forestry, Hunan Collaborat Innovat Ctr Effect Utilizing Woo, Res Inst Wood Ind, 1 Dong Xiao Fu,Xiang Shan Rd, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, 1 Dong Xiao Fu,Xiang Shan Rd, Beijing 100091, Peoples R China;[4]Univ North Texas, Dept Mech & Energy Engn, Denton, TX 76203 USA;[5]Nanjing Forestry Univ, Coll Mat Sci & Engn, 159 Long Pan Rd, Nanjing 210037, Peoples R China

年份：2020

卷号：5

期号：24

起止页码：7164-7169

外文期刊名：CHEMISTRYSELECT

收录：;Scopus(收录号：2-s2.0-85087120612);WOS:【SCI-EXPANDED(收录号:WOS:000544054100004)】；

基金：This research was funded by the National Key Research and Development Program of China (2017YFD0600202), the Research Foundation of Education Bureau of Hunan Province, China (19 C1525) and the Open Foundation of Key Laboratory of Hunan Forest Products and Chemical Industry Engineering (LCHG1905).

语种：英文

外文关键词：C3H-downregulated transgenic poplar; Gene technology; HCT-downregulated transgenic poplar; Lignin structure; Materials science

摘要：Wild type poplar hybrid (Populus alba x P. glandulosacv'84k '),p-coumaroyl shikimate 3 '-hydroxylase (C3H) downregulated transgenic poplar and hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) downregulated transgenic poplar were used as experimental materials. The techniques of two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-HSQC-NMR) spectroscopy and pyrolysis coupled to gas chromatography-mass spectrometry (Py-GC/MS) were used for the analysis and comparison of the lignin structure and monomer ratio between the wild type poplar hybrid and transgenic plants. The results of these analyses showed that the lignin of all poplar samples contained beta-O-4 ' aryl ether linkages and their derivative substructures, resinol substructures, spirodienone substructures, phenylcoumarane substructures,p-hydroxycinnamyl alcohol end groups, syringyl units,p-hydroxyphenyl units, guaiacyl units,p-hydroxybenzoate substructures, and ferulate. Downregulation of C3H and HCT in double transgenic poplar reduced the beta-O-4 ' relative content of lignin in poplar. The S unit, H unit, and S/G ratio in the C3H-downregulated transgenic poplar were increased, while its G unit was decreased. The S unit and S/G ratio in HCT-downregulated transgenic poplar were decreased while its G unit was increased. The C3H-downregulated transgenic poplar was a superior material for biomass energy, pulping, and papermaking than the HCT-downregulated transgenic poplar.