文献类型：期刊文献

中文题名：夏栎茎段离体培养体系的建立

英文题名：Establishment of Isolated Culture System of Quercus robur L.

作者：武荣花[1] 周宗顺[1,2] 任跃[1,2] 李慧[1,2] 陈梦娇[2] 杨海青[3] 朱景乐[2]

第一作者：武荣花

机构：[1]河南农业大学,郑州450003;[2]中国林业科学研究院经济林研究所,经济林种质创新与利用国家林业和草原局重点实验室,郑州450003;[3]河南林业科学研究院,郑州450008

年份：2025

卷号：23

期号：23

起止页码：7932-7938

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：;北大核心:【北大核心2023】；

基金：河南省科技兴林项目“珍贵树种夏橡在沿黄森林城镇建设中应用示范”(YLK202107);河南省科技兴林“欧洲夏橡引种试验示范”(2020);中央级公益性科研项目“栓皮优良种质资源收集与培育技术研究”(CAFYBB2018ZB001-11)共同资助。

语种：中文

中文关键词：夏栎;茎段;诱导;离体培养

外文关键词：Quercus robur L.;Stem segment;Induction;In vitro culture

分类号：S792.18

摘要：为了建立夏栎(Quercus robur L.)器官发生及植株再生体系,以夏栎促萌枝条的幼嫩茎段为外植体,研究消毒方式、基本培养基的种类、外源激素的浓度及其配比等对其再生体系的影响。结果表明,夏栎幼嫩茎段以0.1%氯化汞溶液消毒6 min的成活率最高,为76.66%;基本培养基为WPM时生长状况最佳,在继代培养过程中,6-BA作用明显,以WPM培养基添加0.20 mg/L 6-BA对腋芽诱导分化效果最佳,以WPM培养基添加0.40 mg/L 6-BA对不定芽增殖效果最佳,增殖系数为4.2,接种在含0.25 mg/L IBA+0.10 mg/L NAA的生根培养基,生根率最高,为80.00%。本研究以夏栎一年生茎段为外植体,通过对其消毒、接种、茎段增殖、生根培养,可获得大量组培苗,为夏栎无性繁殖体系的建立、工厂化育苗及基因工程等应用提供理论基础和技术支持。
In order to establish the organogenesis and plant regeneration system of Quercus robur L.,the effects of different disinfection methods,different kinds of basic media,different concentrations of exogenous hormones and their ratio on its regeneration system were studied.The results showed that the highest percentage of success was 76.66%when the young stems of Quercus robur were disinfected with 0.1%mercuric chloride solution for 6 min.The growth status was the better when the basic medium was WPM.In the process of subculture,the effect of 6-BA was obvious.The effect of axillary bud differentiation was the best when 0.20 mg/L 6-BA was added to WPM medium.The effect of adventitious bud proliferation was the best when 0.40 mg/L 6-BA was added to WPM medium.The proliferation coefficient was 4.2.The rooting rate was the highest(80.00%)when the rooting medium was 0.25 mg/L IBA+0.10 mg/L NAA.In this study,the annual stem segments of Quercus robur were used as explants.Through disinfection,inoculation,stem proliferation and rooting culture,a large number of tissue culture seedlings could be obtained,which provided theoretical basis and technical support for the establishment of asexual propagation system,industrialized seedling raising and genetic engineering of Quercus robur.