文献类型：期刊文献

中文题名：欧美杨细菌性溃疡病菌的拮抗细菌筛选鉴定

英文题名：Screening and Identification for Antagonistic Bacteria of Lonsdalea populi

作者：涂艳[1] 韩占府[2] 丁岐峰[3] 薛寒[1] 姜宁[1] 李永[1]

第一作者：涂艳

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所,生物多样性保护国家林业和草原局重点实验室,北京100091;[2]濮阳市农林科学院,濮阳457000;[3]邯郸市森林病虫害防治站,邯郸056002

年份：2024

卷号：4

期号：1

起止页码：66-71

中文期刊名：陆地生态系统与保护学报

外文期刊名：Terrestrial Ecosystem and Conservation

基金：国家自然基金面上项目(31570640);国家菌种资源库项目(NMRC-2023-7)。

语种：中文

中文关键词：欧美杨细菌性溃疡病;杨树朗斯代尔氏菌;多粘类芽孢杆菌;拮抗菌;鉴定

外文关键词：bark canker on Populus×euramericana;Lonsdalea populi;Paenibacillus polymyxa;antagonistic bacteria;identification

分类号：S763.1

摘要：【目的】筛选获得欧美杨细菌性溃疡病菌杨树朗斯代尔氏菌(Lonsdalea populi)的拮抗细菌。【方法】以欧美杨细菌性溃疡病病原菌L.populi CFCC 13731为靶标菌,采用平板拮抗法筛选拮抗菌株,并通过牛津杯法测定其防效。通过观察菌株形态、生理生化实验、16S rRNA序列分析,对高效拮抗菌株进行鉴定。【结果】筛选获得10株具有拮抗作用的菌株,复筛获得1株菌株(k1)对L.populi具有较好的拮抗效果,鉴定结果表明菌株k1为多粘类芽孢杆菌(Paenibacillus polymyxa)。【结论】筛选获得的多粘类芽孢杆菌k1对L.populi具有较好的拮抗作用,在欧美杨细菌性溃疡病防治中将具有较大的应用潜力。
【Objective】The aim is to screen for antagonistic bacteria of Lonsdalea populi which causes the bark canker on Populus×euramericana in China.【Method】The L.populi strain CFCC 13731 was used as target,and the antagonistic strains were screened by the plate antagonism method,their preventive efficacy was determined by the Oxford cup method.The efficient antagonist strains were identified through morphological,physiological and biochemical characteristics in combination with 16S rRNA sequence analysis.【Result】Ten bacteria with antibacterial activity against L.populi were screened.Further screening yielded strain k1 with good antimicrobial activity,which was identified as Paenibacillus polymyxa.【Conclusion】Screening obtained that the P.polymyxa k1 has a good antagonistic effect on L.populi,which will have a greater potential for application in the control of the bark canker on Populus×euramericana.