文献类型：期刊文献

中文题名：柳树β微管蛋白基因家族的克隆和序列分析

英文题名：Cloning and Sequence Analysis of β-Tubulin Gene Families in Willows

作者：睢金凯[1] 饶国栋[1,2] 张建国[1,2]

第一作者：睢金凯

机构：[1]国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]南京林业大学南方现代林业协同创新中心,南京210037

年份：2016

卷号：36

期号：5

起止页码：902-909

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金青年项目(31400569)

语种：中文

中文关键词：柳树;β微管蛋白;系统发育;序列相似性;表达模式

外文关键词：willow; β-tubulin; phylogenetic; sequence similarity; expression pattern

分类号：Q785;Q786

摘要：该研究克隆鉴定了旱柳和龙爪柳β微管蛋白基因,并对其进行了序列相似性、系统发育、染色体定位以及表达模式的分析。结果显示,2种柳树β微管蛋白基因家族各有20个成员,家族内部成员间核酸和氨基酸序列相似性分别在74.0%和86.6%以上,种间同源蛋白氨基酸序列相似性在85.8%以上,柳树与其它植物β微管蛋白间的氨基酸序列相似性在81.5%以上。系统发育分析显示,柳树β微管蛋白家族被分为4个亚组,结合杨树β微管蛋白基因染色体定位,推测柳树β微管蛋白基因家族经历了杨柳科全基因组重复事件和串联重复事件,而柳树TUB11和TUB12可能来源于区段重复或者转座。基因表达模式分析发现,该家族成员的表达具有一定的组织特异性,并且部分重复基因对在所检测组织中表达差异较大。柳树β微管蛋白基因家族成员序列的高度相似性、成员数量的进化扩张、以及表达模式的多样性可能赋予了细胞分裂与生长更高的灵活性,这对多年生木本植物的生长发育习性意义重大。
In this study,β-tubulin genes of Salix matsudana Koidz.and S.matsudana var.tortusoa（Vilm.）Rehd.were cloned and identified.The sequence similarity,phylogeny,chromosomal localization and expression patterns were further analyzed.The result showed that each willow contained 20β-tubulin genes,and each gene shared more than 74.0% and 86.6% nucleotide and amino acid sequence similarity with one another in each willow species.There was over 85.8%amino acid sequence similarity of theβ-tubulin between the two willows.While the amino acid sequence similarity among Salixand other plants were larger than 81.5%.In the phylogenetic analysis,β-tubulin proteins formed four classes,and it was presumed that Salixβ-tubulin gene families had undergone salicoid duplication and tandem duplication events combined with the study of chromosomal location of poplarβ-tubulin.However,TUB11 and TUB12were possibly derived from segmental duplication or replicative transposition.The expression pattern analysis showed a tissue specificity ofβ-tubulin genes in willows,and a section of duplicated-gene pairs showed different expression patterns in tested tissues.High degree of sequence similarity,evolutionary expansionand of members and expression pattern diversity of Salixβ-tubulin gene families might confer flexibility in cell division and growth which is of important significance to the development and growth habit of perennial woody plants.