文献类型：期刊文献

中文题名：石斛菌根化组培苗对P素吸收利用的研究

英文题名：Study on Absorbtion and Exploitation to P Nourishment in the Mycorrhiza of Dendrobium candidum

作者：陈连庆[1] 王小明[1] 裴致达[1]

第一作者：陈连庆

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2005

卷号：18

期号：2

起止页码：163-168

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目:"石斛菌根营养生理的研究"(30170767)部分内容

语种：中文

中文关键词：石斛;菌根化;P营养

外文关键词：Dendrobium candidum ; mycorrhizae; phosphorus nutrition

分类号：S722.37

摘要：濒危石斛菌根化吸收、积累P素营养的研究结果表明:可溶性和难溶性不同P源,对菌根化石斛组培苗生长的效应不同,试验处理3(KH2PO4)和处理6(FePO4)的干质量分别为0.018、0.019g,是处理1(K2HPO4)的2.62倍和2.78倍,差异达到极显著水平。可溶性P(KH2PO4)不同浓度对菌根化石斛组培苗的综合效应,表现在苗高、径粗和干质量等主要指标上,处理3为最适宜质量浓度(0.25g·L-1),与处理9(CK)相对比,苗高是2.64倍、径粗是2.16倍和干物质是3.87倍。对苗木N、C、P营养元素的吸收、积累分析,处理3是CK的1.72、1.40和11.53倍;不同有效P含量对石斛菌根真菌F9903菌株增殖生长的试验表明:当培养液浓度为含纯P为1mg×22.76%时,其菌丝体增殖效果最佳,干质量高达1.256mg·mL-1,是接种量的114.2倍。不同P浓度对菌根化石斛组培苗干质量的影响及其对共生菌根真菌菌丝体干质量的影响两个柱型图的重叠性好,其图形近似,效果趋势一致。
The paper dealed with the absorbtion and accumulation P nourishmet in mycorrhiza of Dendrobium candidum.The results showed that the effect of P with different origin to the seedling growth was different. The dry weights of treatment 3( KH_2PO_4) and treatment 6( FePO_4) were 0.018 and 0.019 g respectively, which were 2.62 and 2.78 times than that of treatment 1. The difference was significant. The effect of different concentration of dissoluble P(KH_2PO_4) to the mycorrhiza tissue culture seedling of Dendrobium candidum, primarily expressed in seedling height, stem and dry weight etc. Treatment 3 was the most suitable and proper density was 0.25 g· L^(-1). Compared with treatment 9(CONTROL), the seedling height, stem and dry weight of treatment 3 were 2.64, 2.16 and 3.87 times respectively. Through the analysis to the N, C, P nutrient absorption and accumulation, treatment 3 was 1.72, 1.40 and 11.53 times than that of CONTROL. The result of test on the effect of different available P to propagational growth of Dendrobium candidum mycorrhiza fungi F9903 showed that, when the culture solution contained 1 mg of P content was 22.76% in KH_2PO_4, the effect of mycelium multiplication was the best, the dry weight was up to 1.256 mg·mL^(-1) ,114.2 times that of its inoculum size. In the pillar type diagram of the dry weight of the symbiosis mycorrhiza fungi hypha and the tissue culture seedling with mycorrhiza of Dendrobium candidum in the different density of P nourishment, the shape of diagram and the trend were similar.