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8种杀菌剂对9种薄壳山核桃炭疽病病原菌的毒力测定     被引量:12

Toxicity Test with 8 Fungicides Against 9 Pathogens of Pecan Anthracnose(Colletotrichum spp.)

文献类型:期刊文献

中文题名:8种杀菌剂对9种薄壳山核桃炭疽病病原菌的毒力测定

英文题名:Toxicity Test with 8 Fungicides Against 9 Pathogens of Pecan Anthracnose(Colletotrichum spp.)

作者:孟珂[1,2] 张亚波[2] 常君[2] 李志红[1] 王迪[1] 翟凤艳[1] 舒金平[2]

第一作者:孟珂

机构:[1]河南科技学院,河南新乡453003;[2]中国林业科学研究院亚热带林业研究所,浙江杭州311400

年份:2021

卷号:34

期号:1

起止页码:153-164

中文期刊名:林业科学研究

外文期刊名:Forest Research

收录:CSTPCD;;Scopus;北大核心:【北大核心2020】;CSCD:【CSCD2021_2022】;

基金:中国林科院基本科研业务费专项资金项目(CAFYBB2019ZB002);浙江省省院合作林业科技项目(2017SY18)。

语种:中文

中文关键词:薄壳山核桃;炭疽病;杀菌剂;毒力测定

外文关键词:Carya illinoensis;anthracnose;fungicide;toxicity test

分类号:S763.1

摘要:[目的]测定8种杀菌剂对9种炭疽菌(26个菌株)的室内毒力,筛选出防治薄壳山核桃炭疽病的高效药剂。[方法]采用菌丝生长速率法测定8种杀菌剂对26株炭疽菌菌丝生长的抑制作用,构建毒力回归方程,并计算EC50值。[结果]供试8种杀菌剂中,咪鲜胺和咯菌腈对所分离的9种炭疽菌(26个菌株)菌丝生长的抑制作用最强,平均EC50值分别为0.14、0.15 mg·L^(?1)。戊唑醇对C.fioriniae、C.liaoningense、C.tamarilloi和C.americae-borealis菌丝生长的抑制作用较强(平均EC50=0.49 mg·L^(?1)),但对另5种炭疽菌效果不明显(平均EC50=23.60 mg·L^(?1))。恶醚唑对C.nymphaeae、C.siamense、C.alienum、C.fioriniae和C.coelogynes菌丝生长的抑制作用较强(平均EC50=0.81 mg·L^(?1)),但对另4种炭疽菌抑制效果一般(平均EC50=2.00 mg·L^(?1))。代森锰锌对9种病原菌菌丝生长的抑制性最差,平均EC50值为42.70 mg·L^(?1)。[结论]咪鲜胺、咯菌腈对薄壳山核桃炭疽菌具有较高的毒力,在薄壳山核桃炭疽病林间防治中具有较大的应用潜力。为避免产生抗药性,建议轮换使用咪鲜胺、咯菌腈、恶醚唑和甲基硫菌灵等杀菌剂。
[Objective]To test the toxicity of 8 fungicides against Colletotrichum in laboratory and screen out effective fungicides to control pecan anthracnose.[Method]The method of comparing mycelia growth rate was used to determine the fungistasis of 8 fungicides to 26 strains of 9 Colletotrichum species,then the virulence regression equations were built,and the EC50 values for each fungicide were calculated.[Result]Among the 8 fungicides tested,Prochloraz and Fludioxonil showed the strongest fungistasis to mycelium growth of all trains,with an average EC50 value of 0.14 mg·L-1 and 0.15 mg·L-1,respectively.Tebuconazole had a strong fungistasis to mycelium growth of C.fioriniae,C.liaoningense,C.tamarilloi and C.americae-borealis(mean EC50=0.49 mg·L^(?1)),but showed a moderate inhibitory effect on other 5 species(mean EC50=23.60 mg·L^(?1)).Difenoconazole had a strong inhibitory effect on mycelium growth of five Colletotrichum species,C.nymphaeae,C.siamense,C.alienum,C.fioriniae and C.coelogynes(mean EC50=0.81 mg·L^(?1)),but showed low virulence for the other 4 species(mean EC50=2.00 mg·L^(?1)).The virulence of Mancozeb for the 9 pathogens was the worst,with the average EC50 value of 42.70 mg·L^(?1).[Conclusion]Prochloraz and Fludioxonil show high virulence for pecan anthracnose,and are the optimal candidates for field control against pecan anthracnose.It is suggested to use Prochloraz,Fludioxonil,Difenoconazole and Thiophanate-methyl in rotation to control pecan anthracnose effectively and avoid developing resistance.

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