文献类型：期刊文献

中文题名：低温胁迫下鹅掌楸抗寒性相关基因的差异表达分析

英文题名：Analysis on differential expression of cold resistance related genes of Liriodendron chinense under low temperature stress

作者：陆畅[1] 李斌[1] 郑勇奇[1]

第一作者：陆畅

机构：[1]国家林木遗传育种重点实验室中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2015

卷号：24

期号：3

起止页码：25-31

中文期刊名：植物资源与环境学报

外文期刊名：Journal of Plant Resources and Environment

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金："十二五"国家科技支撑计划项目(2013BAD01B06)

语种：中文

中文关键词：鹅掌楸;低温胁迫;抗寒性相关基因;数字基因表达谱;差异表达基因;相对表达量

外文关键词：Liriodendron chinense （Hemsl.） Sarg.; low temperature stress ; cold resistance related gene ;digital gene expression profile; differential expression gene; relative expression

分类号：Q948.112.2;Q946-33

摘要：对4℃和-30℃低温条件下引种自安徽大别山的1株鹅掌楸〔Liriodendron chinense（Hemsl.）Sarg.〕植株顶芽总RNA进行了数字基因表达谱构建和差异表达基因筛选,并对筛选出的抗寒性相关基因进行了实时荧光定量PCR分析。结果表明：获得的总RNA样品质量符合实验要求。数字基因表达谱分析结果表明：每个样品的质量控制后序列的总长度均在0.5 Gbp以上,碱基错误率为0.01%,Q20值超过99%,Q30值为96.99%~97.23%,GC含量为44.97%~47.06%,并且每个样品比对成功的序列条数占质量控制后序列总数的百分率均在90%以上。差异表达基因筛选结果显示：共发现9个与鹅掌楸抗寒性有关的差异表达基因,分别为HSP、MYB、NAC、AP2、Zinc finger、WRKY、FAD、Phospholipase和β-amylase基因。实时荧光定量PCR分析结果表明这9个基因共有3种表达模式,其中,HSP和FAD基因的相对表达量均随着温度的降低而减少,表现为基因下调表达;AP2、NAC和Zinc finger基因的相对表达量随着温度的降低而增多,表现为基因上调表达;而β-amylase、WRKY、Phospholipase和MYB基因的相对表达量则表现为4℃时增加、-30℃时减少。从基因的相对表达量来看,最大相对表达量超过5的基因有NAC和WRKY,介于2~5之间的基因有AP2、Zinc finger、β-amylase和MYB,低于2的基因有Phospholipase、HSP和FAD。结果显示NAC和WRKY基因可能在鹅掌楸的抗寒过程中起主要作用。
Under 4 ℃ and -30 ℃ low temperature conditions, digital gene expression profile of total RNA from apical buds of one individual of Liriodendron chinense （Hemsl.） Sarg. introduced from Dabieshan of Anhui was constructed and differential expression genes were selected, and cold resistance related genes selected were analyzed by real-time fluorescence quantitative PCR. The results show that qualities of total RNA samples obtained meet the experimental requirements. Analysis results of digital gene expression profile show that total length of reads after quality control of each sample is above 0.5 Gbp, error rate of base is 0.01% , Q20 value is more than 99% , Q30 value is 96.99% -97.23% , GC content is 44.97% -47.06% , and percentage of successfully mapped read number to total read number after quality control of each sample is above 90%. Selection result of differential expression genes shows that nine differential expression genes related to cold resistance of L. chinerue are founded totally, they are HSP, MYB, NAC, AP2, Zinc finger, WRKY, FAD, Phospholipase and fl-amylase genes, respectively. Real-time fluorescence quantitative PCR analysis result shows that these nine genes have three expression modes, in which, relative expressions of HSP and FAD genes both decrease with decreasing of temperature, appearing gene down-regulated expression; those of AP2, NAC and Zinc finger genes increase with decreasing of temperature, appearing gene up-regulated expression ; while those of βE-amylase, WRKY, Phospholipase and MYB genes appear increasing at 4 ℃ and decreasing at -30 ℃. On the view of gene relative expression, genes with maximum relative expression over 5 are NAC and WRKY, genes with that between 2-5 are AP2, Zinc finger, ℃-amylase and MYB, and genes with that under 2 are Phospholipase, HSP and FAD. It is suggested that NAC and WRKY genes may play a major role in the process of cold resistance of L. chinense.