文献类型：期刊文献

英文题名：Unlocking rhizosphere phosphorus: Root exudate-microbe synergy drives phosphorus activation in mixed Chinese fir species plantation

作者：Ding, Yijie[1] Yao, Jiabao[2] Li, Fengqing[2] Hou, Lingyu[1] Cheng, Jie[1] Zhang, Runzhe[1] Dong, Yuhong[1] Liu, Lei[1] Sun, Qiwu[1]

第一作者：Ding, Yijie

机构：[1] State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Experimental Center of Subtropical Forestry, Chinese Academy of Forestry, Jiangxi, China

年份：2025

卷号：237

外文期刊名：Industrial Crops and Products

收录：EI(收录号：20254519455171);Scopus(收录号：2-s2.0-105020819292)

语种：英文

外文关键词：Cultivation - FIR filters - Forestry - Genes - Nutrients - Phosphorus - Plants (botany) - Polonium alloys - Polonium compounds - Solubility - Tropics

摘要：Cunninghamia lanceolata (Lamb.) Hook., also termed as Chinese fir, is a rapidly growing plantation species of significant economic value in China. However, long-term pure plantations have exacerbated soil phosphorus (P) limitations by reducing both P availability and turnover. Mixed-species plantations offer a promising strategy to enhance nutrient availability, yet the underlying mechanisms by which they alleviate soil P limitation remain largely unexplored. This study conducted a 15-year transformation experiment in subtropical China and found that interplanting Schima superba with Chinese fir significantly increased rhizosphere P availability. Sequential P fractionation revealed a notable shift from stable P forms to moderately labile and labile and P pools in mixed plantations, with NaHCO3-Po increasing by 108 % and NaOH-Po by 58.3 % compared to pure plantations. Metabolomic analysis identified four organic acids, oxoproline, alanine-2, aconitic acid, and N-carbamylglutamate-4 (NCG 4), secreted exclusively by Chinese fir roots in the mixed-species system. These root exudates were strongly correlated with NaHCO3-Po levels and activated a microbial P-solubilization gene network. This included upregulation of inorganic P-solubilizing genes (ppk, ppx, and pqqD), high-affinity phosphate transporters (pstA, pstB, and pstC), and P-starvation response regulators (phoB, phoP, and phoU). Partial least squares path explaining 99 %, 91 %, and 81 % of the variations in labile, moderately labile, and stable phosphorus fractions and modeling further indicated that changes in rhizosphere P fractions were driven by the interactions among root exudates, microbial functional genes, and environmental factors. Together, these findings support a synergistic "root exudate-microbial gene" mechanism that transforms stable P forms into plant-available P, offering a blueprint for designing rhizosphere-engineered mixed-species plantations to overcome P limitation and promote sustainable forestry. ? 2025