文献类型：期刊文献

英文题名：Transporters and ascorbate-glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes

作者：Han, Xiaojiao[1,2] Zhang, Yunxing[1,2,3] Yu, Miao[1,2] Zhang, Jin[4] Xu, Dong[1,2] Lu, Zhuchou[1,2] Qiao, Guirong[1,2] Qiu, Wenmin[1,2] Zhuo, Renying[1,2]

第一作者：韩小娇

通信作者：Zhuo, RY[1];Zhuo, RY[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]Henan Polytech Univ, Sch Architectural & Artist Design, Jiaozuo 454000, Henan, Peoples R China;[4]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

年份：2020

卷号：40

期号：8

起止页码：1126-1142

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85087662956);WOS:【SCI-EXPANDED(收录号:WOS:000591568200012)】；

基金：Funding for this work was provided by Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2016C02056-1), the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2017ZY007), the National Transformation Science and Technology Program (2018ZX08020002-005-003), the National Natural Science Foundation of China (31872168) and Key Technologies R&D Program of Henan Province (182102310052).

语种：英文

外文关键词：accumulation and detoxification; AsA-GSH metabolism pathway; cadmium stress; comparative morphophysiology and transcriptome; Salix matsudana var. matsudana f. umbraculifera

摘要：Salix matsudana Koidz is a low cadmium (Cd)-accumulating willow, whereas its cultivated variety, Salix matsudana var. matsudana f. umbraculifera Rehd., is a high Cd-accumulating and tolerant willow (HCW). The physiological and molecular mechanisms underlying differential Cd accumulation and tolerance in the two Salix species are poorly understood. Here, we confirmed that the differential Cd translocation capacity from roots to the shoots leads to the differential Cd accumulation in their aboveground parts between these two willow genotypes. Cadmium accumulation happens preferentially in the transport pathway, and Cd is mainly located in the vacuolar, cell wall and intercellular space in HCW bark by cadmium location analysis at tissue and subcellular levels. Comparative transcriptome analysis revealed that higher expressions of several metal transporter genes (ATP-binding cassette transporters, K+ transporters/channels, yellow stripe-like proteins, zinc-regulated transporter/iron-regulated transporter-like proteins, etc.) are involved in root uptake and translocation capacity in HCW; meanwhile, ascorbate-glutathione metabolic pathways play essential roles in Cd detoxification and higher tolerance of the Cd-accumulator HCW. These results lay the foundation for further understanding the molecular mechanisms of Cd accumulation in woody plants and provide new insights into molecular-assisted-screening woody plant varieties for phytoremediation.