文献类型：期刊文献

英文题名：MiR319a-mediated salt stress response in poplar

作者：Cheng, Yanxia[1] Wang, Qiao[2] Yang, Linxi[1] Li, Quanzi[1] Yan, Xiaojing[1]

第一作者：Cheng, Yanxia

通信作者：Li, QZ[1];Yan, XJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Shandong, Peoples R China

年份：2024

卷号：11

期号：8

外文期刊名：HORTICULTURE RESEARCH

收录：;Scopus(收录号：2-s2.0-85200908963);WOS:【SCI-EXPANDED(收录号:WOS:001283870700005)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFD2200900 to X.Y.), Fundamental Research Funds of SKLTGB (CAF) (TGBFRF202301 to X.Y.). We thank Prof. Dayong Li (Beijing Academy of Agriculture and Forestry Sciences) and Prof. Wanjun Zhang (China Agricultural University) for sharing plasmids pZH01: OsamiR319a-OE and miR319a-MIMIC. We thank Prof. Jinshan Gui (Zhejiang Agriculture and Forestry University) for sharing plasmids pCAMBIA2300:PdXCP1p-35Smini-GUS and pCAMBIA2300:PdDUF579-9p-35Smini-GUS. We thank Shuai Liu and Dandan Yin (State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing) for their technical assistance for ICP-MS and SEM analyses.

语种：英文

摘要：Maintenance of intracellular ion balance, especially Na+ and K+, plays an important role in plant responses to salt stress. Vessels in xylem are responsible for long-distance ion transport in vascular plants. Knowledge on the salt stress response in woody plants in limited. In this study, we identified miR319a as an important regulator in respond to salt stress in poplar. miR319a overexpression transgenic poplar showed a salt-tolerant phenotype, and cytological observation showed reduced cambium cell layers, wider xylem, increased number and lumen area of vessels and fibers, and thinner cell wall thickness in the transgenics. The miR319a-MIMIC plants, meanwhile, had opposite phenotypes, with narrower xylem, reduced number and lumen area of vessels and fibers cells, and increased wall thickness. In addition, overexpression of miR319a driven by the vessel-specific promoter significantly improved the salt tolerance compared with the fiber-specific promoter. The expression levels of PagHKT1;2 and PagSKOR1-b, which encoded high-affinity K+ and Na+ transporters for Na+ efflux and K+ influx, respectively, were positively correlated with the vessel number and lumen area. These results suggest that miR319 not only promotes ion transport rates by increasing vessel number and lumen area and reducing cell wall thickness, but also regulates the concentrations of Na+ and K+ in the xylem by up-regulating PagHKT1;2 and PagSKOR1-b. We demonstrate that miR319 may coordinate the response of poplar to salt stress through both mechanisms, enriching our understanding of the synergistic effects of the secondary xylem structure and long-distance ion transport balance in the salt tolerance of poplar.