详细信息
松墨天牛纤维素酶的研究 Ⅱ.离体条件下金属离子对松墨天牛纤维素酶活性的影响 被引量:11
Study on the Character of Cellulase in Monochamus alternatus Ⅱ.Effect of Some Ions on the Monochamus alternatus Cellulase Activity in Vitro
文献类型:期刊文献
中文题名:松墨天牛纤维素酶的研究 Ⅱ.离体条件下金属离子对松墨天牛纤维素酶活性的影响
英文题名:Study on the Character of Cellulase in Monochamus alternatus Ⅱ.Effect of Some Ions on the Monochamus alternatus Cellulase Activity in Vitro
作者:索风梅[1] 王浩杰[1] 陈洪宝[2] 徐天森[1]
第一作者:索风梅
机构:[1]中国林业科学研究院亚热带林业研究所;[2]浙江省富阳市林业局
年份:2006
卷号:19
期号:2
起止页码:205-210
中文期刊名:林业科学研究
外文期刊名:Forest Research
收录:CSTPCD;;Scopus;北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;
基金:浙江省自然科学基金项目"松墨天牛消化酶演变规律和抑制效应的研究"(303318)
语种:中文
中文关键词:松墨天牛;离体条件;金属离子;纤维素酶系;酶活性
外文关键词:Monochamus alternatus ; invitro ; ions ; cellulose ; activity
分类号:S718.7
摘要:本文研究了Cu^++、Mn^++、Ca^++、Pb^++、Ag^+、Zn^++、Na^+、Fe^++8种金属离子对松墨天牛(幼虫、成虫)消化道纤维素酶活性(离体条件下)的影响。结果表明:不同的金属离子对纤维素酶有不同程度的影响,除Fe^++有激活作用外,其它7种金属离子对纤维素酶各组分酶活性在实验所取浓度范围内(0.1~50mmol·L^-1)表现为程度不同的抑制作用,其中Cu^++和Pb^++的抑制效应最强,这在纤维素酶的组分(Cx酶、β-1,4-葡萄糖苷酶、C1酶)都有很鲜明的体现,与对照相比较,在不同浓度梯度下使Cx酶的活性下降13.19%-99.98%42.27%~100%。
The effects of eight ions on the Monochamus alternatus cellulase activity in vitro were studied in this paper. The results indicated that the activity reduced differently when concentration of ions were added except Fe^++ in experiment conditions. Among which the effects of Cu ^++ and Pb^++ were obvious on cellulolytic enzyme complex : endo-β-1,4-glucanase (Cx-ase), exo-β-1,4-glucanase (C1-ase) and β-1,4-glucosidase in their gut, and activity had declined by 13.19% - 99.98% and 42.27%- 100% respectively under different concentration gradients.
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