文献类型：期刊文献

英文题名：Assembly and phylogenetic analysis of the complete mitochondrial genome of a widely planted hybrid eucalypt (Eucalyptus urophylla × E. grandis)

作者：Li, Chenhe[1] Wang, Jing[1] Fan, Chunjie[2] Kang, Xiangyang[1] Yang, Jun[1]

第一作者：Li, Chenhe

机构：[1] State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; [2] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of State Forestry and Grassland Administration On Tropical Forestry, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China

年份：2025

卷号：36

期号：1

外文期刊名：Journal of Forestry Research

收录：EI(收录号：20252018431242);Scopus(收录号：2-s2.0-105005026387)

语种：英文

外文关键词：Algae - Genetically modified plants - Mycelium - Nanopores - Plant extracts - Vegetation

摘要：Eucalyptus urophylla × E. grandis is a major hybrid species of timber plantations. However, our understanding of Eucalyptus mitochondrial genome, especially within the Myrtaceae family, is limited. In this study, we employed hybrid sequencing combining the Illumina and Oxford Nanopore sequencing to assemble and annotate the mitogenome (mtDNA) of E. urophylla × E. grandis. Our results reveal a structure characterized by one circular molecule, with a cumulative length of 483,907 base pairs (bp) and a GC content of 44.96%. The circular molecule collectively harbored 59 annotated genes. Among these, 38 were unique protein-coding genes (PCGs), accompanied by 18 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. Our study also examined repetitive sequences, RNA editing sites, and intracellular sequence transfers within the mtDNA. Furthermore, we conducted a phylogenetic analysis between E. urophylla × E. grandis and 30 closely related species based on genetic affinities. The outcomes furnish a high-quality organelle genome for E. urophylla × E. grandis, thereby explaining basic insights into organelle genome evolution and phylogenetic relationships. ? Northeast Forestry University 2025.