文献类型：期刊文献

英文题名：Exogenous Abscisic Acid Modulates Reactive Oxygen Metabolism and Related Gene Expression in Platycladus orientalis under H2O2-Induced Stress

作者：Yao, X. M.[1,2] Ji, J.[2] Yue, J. Y.[2] Shi, S. Q.[2] Ou, C.[1] Jiang, Z. P.[2] Chang, E. M.[2]

第一作者：Yao, X. M.

通信作者：Jiang, ZP[1];Chang, EM[1]

机构：[1]Fuyang Normal Univ, Engn Technol Res Ctr Antiaging Chinese Herbal Med, Biol & Food Engn Sch, Fuyang 236037, Anhui, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：67

期号：1

起止页码：85-93

外文期刊名：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519881300008)】；

基金：This research was supported by grants from the National Natural Science Foundation of China (project no. 31870664), the National Non-Profit Research Institutions of the Chinese Academy of Forestry (project no. CAFYBB2017ZA007), the Natural Science Key Foundations of the Anhui Bureau of Education (project no. KJ2018A0349), the Open Fund Projects of Zhejiang Provincial Key Laboratory of Plant Pest Control (project no. 2010DS700124-KF1816), the Natural Science Key Foundations of Fuyang Normal University (project no. 2018FSKJ03ZD), and the Young Talent Key Foundations of Fuyang Normal University (project no. rcxm201806).

语种：英文

外文关键词：Platycladus orientalis; H2O2-induced stress; ABA; reactive oxygen metabolism; gene expression

摘要：Abscisic acid (ABA) is a plant hormone that regulates tolerance to abiotic stress. In this study, we evaluated the effect of exogenous different concentrations of ABA (0, 0.5, 1, 10, 100 and 200 mu M) to mitigate oxidative stress in Platycladus orientalis (L.) Franco exposed to 100 mM hydrogen peroxide (H2O2), and its effects on reactive oxygen metabolism. Results indicated that exposure to 100 mM H2O2 significantly increased the contents of H2O2, malondialdehyde (MDA), ascorbic acid, total glutathione, and proline, and the activities of superoxide dismutase (SOD) and catalase (CAT) in P. orientalis leaves, while peroxidase (POD) activity decreased. The transcript levels of Cu/Zn-SOD, CAT, GR, APX, MDAR, and GST were upregulated in P. orientalis after 48 h of H2O2-induced stress. Compared with 100 and 200 mu M ABA, lower ABA concentrations (0.5, 1, and 10 mu M) significantly enhanced the activities of SOD, POD and CAT, and increased the contents of ascorbic acid, total glutathione, and proline in H2O2-treated seedlings at 48 h, accompanied by reduced H2O2 and MDA contents. Among the three lower concentrations of ABA, the 1 mu M ABA concentration had the strongest effect on enzyme activities and the contents of antioxidant compounds. Among all the ABA concentrations, 1 mu M ABA had the strongest effect to increase the transcript levels of Cu/Zn-SOD, CAT, GR, APX and MDAR in P. orientalis under H2O2-induced stress. This study revealed that an appropriate concentration of ABA effectively mitigated the damage caused by reactive oxygen species to P. orientalis via regulating the antioxidant defense system.