文献类型：期刊文献

英文题名：Whole-genome re-sequencing reveals molecular mechanisms of biomass changes in 11-year-old Bt transgenic poplar

作者：Zhang, Jin[1] Zhan, Tian-Yi[2] Jia, Hui-Xia[1] Wu, Li-Shuan[1] Lu, Meng-Zhu[1] Hu, Jian-Jun[1]

第一作者：Zhang, Jin

通信作者：Hu, Jian-Jun|[a0005f77eede4dc147cfc]胡建军;

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China

年份：2018

卷号：32

期号：6

起止页码：1609-1620

外文期刊名：Trees - Structure and Function

收录：EI(收录号：20182905568175);Scopus(收录号：2-s2.0-85049946229)

语种：英文

外文关键词：Chemical analysis - Wood - Cell death - Proteins - Genes

摘要：Key message: Genome-wide insertions/deletions were identified in Bt transgenic poplar through whole-genome re-sequencing, revealing the molecular mechanisms underlying changes in the growth and wood characteristics of Bt transgenic poplar. Abstract: Poplar is a fast-growing tree that relieves the wood demand in China. Previously, we found growth and wood characteristics differed in some lines of insect-resistant Bt transgenic poplar. Here, we selected five Bt transgenic lines (#12, #153, #172, #208 and #209), one untransformed control (CK2), one comparable control (CK3), and one native cultivar (JY, Populus × euramericana ‘Robusta’) in Xinjiang, China, for further study. Based on breast diameter, tree height, chemical content, and fibre morphology analyses, these transgenic lines showed fast growth and high-lignin and low-cellulose contents in the cell wall. In addition, genome-wide insertions/deletions (InDels) were compared between two transgenic lines (#12 and #153) and the untransformed control via whole-genome re-sequencing. GO enrichment and protein–protein interaction networks indicated the genes affected by differential InDels were related to programmed cell death and defence responses. This study provides new insight into the molecular mechanisms underlying changes in biomass characteristics in Bt transgenic poplar. ? 2018, Springer-Verlag GmbH Germany, part of Springer Nature.