文献类型：期刊文献

英文题名：Genome-Wide Identification and Functional Analysis of the GUX Gene Family in Eucalyptus grandis

作者：Li, Linsi[1] Tang, Jiye[1] Wu, Aimin[1] Fan, Chunjie[2] Li, Huiling[1]

第一作者：Li, Linsi

通信作者：Li, HL[1];Fan, CJ[2]

机构：[1]South China Agr Univ, Coll Forestry & Landscape Architectures, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Key Lab State Forestry & Grassland Adm Trop Forest, Guangzhou 510520, Peoples R China

年份：2024

卷号：25

期号：15

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85200985509);WOS:【SCI-EXPANDED(收录号:WOS:001287861700001)】；

基金：The author(s) declare financial support was received for the research, authorship, and/orpublication of this article. This study was supported by Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm; College of Forestry and Landscape Architectures; South China Agricultural University, Guangzhou 510642; China and State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of State Forestry and Grassland Administration on Tropical Forestry; Research Institute of Tropical Forestry; Chinese Academy of Forestry,Guangzhou 510520, China; and the Innovation and Entrepreneurship training program for college students. This paper was supported by Guangzhou Science and Technology Project (202201011282),China Postdoctoral Science Foundation Project (2022M721198), Guangdong Natural Science Foundation Project (2023A1515010379), and National Innovation and Entrepreneurship Competition for College Students.

语种：英文

外文关键词：GUX family genes; Eucalyptus grandis; genome-wide identification; functional analysis

摘要：Xylan, one of the most important structures and polysaccharides, plays critical roles in plant development, growth, and defense responses to pathogens. Glucuronic acid substitution of xylan (GUX) functions in xylan sidechain decoration, which is involved in a wide range of physiological processes in plants. However, the specifics of GUXs in trees remain unclear. In this study, the characterization and evolution of the GUX family genes in E. grandis, a fast-growing forest tree belonging to the Myrtaceae family, were performed. A total of 23 EgGUXs were identified from the E. grandis genome, of which all members contained motif 2, 3, 5, and 7. All GUX genes were phylogeneticly clustered into five distinct groups. Among them, EgGUX01 similar to EgGUX05 genes were clustered into group III and IV, which were more closely related to the AtGUX1, AtGUX2, and AtGUX4 members of Arabidopsis thaliana known to possess glucuronyltransferase activity, while most other members were clustered into group I. The light-responsive elements, hormone-responsive elements, growth and development-responsive elements, and stress-responsive elements were found in the promoter cis-acting elements, suggesting the expression of GUX might also be regulated by abiotic factors. RNA-Seq data confirmed that EgGUX02, EgGUX03, and EgGUX10 are highly expressed in xylem, and EgGUX09, EgGUX10, and EgGUX14 were obviously responses to abiotic stresses. The results of this paper will provide a comprehensive determination of the functions of the EgGUX family members, which will further contribute to understanding E. grandis xylan formation.