文献类型：期刊文献

英文题名：Deciphering Small Noncoding RNAs during the Transition from Dormant Embryo to Germinated Embryo in Larches (Larix leptolepis)

作者：Zhang, Junhong[1,2] Zhang, Shougong[2] Han, Suying[3] Li, Xinmin[2,4] Tong, Zaikang[1] Qi, Liwang[2]

第一作者：Zhang, Junhong

通信作者：Tong, ZK[1]

机构：[1]Zhejiang Agr & Forestry Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China;[4]Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA USA

年份：2013

卷号：8

期号：12

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000328707400018)】；

基金：This work was supported by the National High Technology Research and Development Program of China (grant nos. 2011AA100203, 2013AA102704) and the National Basic Research Program of China (grant no. 2009CB119106). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Small RNAs (sRNAs), as a key component of molecular biology, play essential roles in plant development, hormone signaling, and stress response. However, little is known about the relationships among sRNAs, hormone signaling, and dormancy regulation in gymnosperm embryos. To investigate the roles of sRNAs in embryo dormancy maintenance and release in Larix leptolepis, we deciphered the endogenous "sRNAome'' in dormant and germinated embryos. High-throughput sequencing of sRNA libraries showed that dormant embryos exhibited a length bias toward 24-nt while germinated embryos showed a bias toward 21-nt lengths. This might be associated with distinct levels of RNA-dependent RNA polymerase2 (RDR2) and/or RDR6, which is regulated by hormones. Proportions of miRNAs to nonredundant and redundant sRNAs were higher in germinated embryos than in dormant embryos, while the ratio of unknown sRNAs was higher in dormant embryos than in germinated embryos. We identified a total of 160 conserved miRNAs from 38 families, 3 novel miRNAs, and 16 plausible miRNA candidates, of which many were upregulated in germinated embryos relative to dormant embryos. These findings indicate that larches and possibly other gymnosperms have complex mechanisms of gene regulation involving miRNAs and other sRNAs operating transcriptionally and posttranscriptionally during embryo dormancy and germination. We propose that abscisic acid modulates embryo dormancy and germination at least in part through regulation of the expression level of sRNA-biogenesis genes, thus changing the sRNA components.