文献类型：期刊文献

英文题名：Phylogenetic tree-informed microRNAome analysis uncovers conserved and lineage-specific miRNAs in Camellia during floral organ development

作者：Yin, Hengfu[1,2] Fan, Zhengqi[1,2] Li, Xinlei[1,2] Wang, Jiangying[1] Liu, Weixin[1] Wu, Bin[1,2] Ying, Zhen[1] Liu, Liping[1] Liu, Zhongchi[3] Li, Jiyuan[1,2]

第一作者：Yin, Hengfu

通信作者：Yin, HF[1];Li, JY[1];Yin, HF[2];Li, JY[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[2]Key Lab Forest Genet & breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

年份：2016

卷号：67

期号：9

起止页码：2641-2653

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000376385800012)】；

基金：We acknowledge funding from Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (No. 2012BAD01B07). This work was supported by the CAF Nonprofit Research Projects (RISF61250) and National Natural Science Foundation of China (grant no. 31470697) and Breeding New Flower Varieties Program of Zhejiang Province (2012C12909-6). We thank Biomarker technologies (Beijing, China) for the sequencing work. We are grateful to Professor Philip Donoghue from the University of Bristol (UK) for help with data analysis.

语种：英文

外文关键词：Camellia; differential expression; floral development; lineage specific; microRNA identification; miRNA-target regulation

摘要：In plants, miRNAs are endogenous small RNAs derived from single-stranded precursors with hairpin structures. The evolution of miRNAs and their targets represents one of the most dynamic circuits directing gene expression, which may play fundamental roles in shaping the development of distinct plant organs. Here we performed high-throughput small RNA sequencing in five organ types of Camellia azalea to capture the spatial profile of small non-coding RNA. In total we obtained > 227 million high-quality reads and identified 175 miRNAs with mature and precursor sequences. We aligned the miRNAs to known miRNA databases and revealed some conserved as well as 'newly evolved' miRNA genes. Twelve miRNAs were identified to be specific in the genus Camellia, supporting the lineage-specific manner of expansion of 'young' miRNAs. Through differential expression analysis, we showed that many miRNAs were preferentially abundant in certain organ types. Moreover, hierarchical clustering analysis revealed distinctive expression patterns of tissue-specific miRNAs. Gene Ontology enrichment analysis of targets of stamen- and carpel-specific miRNA subclusters showed that miRNA-target regulatory circuits were involved in many important biological processes, enabling their proper specification and organogenesis, such as 'DNA integration' and 'fruit development'. Further, quantitative PCR of key miRNAs and their target genes revealed anti-correlated patterns, and uncovered the functions of key miRNA-target pairs in different floral organs. Taken together, this work yielded valuable information on miRNA-target regulation in the control of floral organ development and sheds light on the evolution of lineage-specific miRNAs in Camellia.