文献类型：期刊文献

英文题名：Chalcone isomerase in grape vine: gene expression and localization in the developing fruit

作者：Wang, W.[1,3] Wang, H. -L.[1,2] Wan, S. -B.[1,4] Zhang, J. -H.[1] Zhang, P.[1] Zhan, J. -C.[1] Huang, W. -D.[1,2]

第一作者：Wang, W.;王伟

通信作者：Huang, WD[1]

机构：[1]China Agr Univ, Ctr Res & Dev Viticulture & Enol, Beijing 100083, Peoples R China;[2]Beijing Key Lab Viticulture & Enol, Beijing 100083, Peoples R China;[3]Chinese Acad Forestry, Inst Forestry, Beijing 100091, Peoples R China;[4]Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China

年份：2012

卷号：56

期号：3

起止页码：545-550

外文期刊名：BIOLOGIA PLANTARUM

收录：;Scopus(收录号：2-s2.0-84859879060);WOS:【SCI-EXPANDED(收录号:WOS:000303062400019)】；

基金：This research was supported by major program of Beijing Municipal Science & Technology Commission (No. D07060500160701) and China Postdoctoral Science Foundation funded project (No. 20090450319). The first two authors contribute equally to this work.

语种：英文

外文关键词：antibody; Escherichia coli; flavonoids; immunohistochemical technique; RT-PCR; Vitis vinifera

摘要：Chalcone isomerase (CHI, EC 5.5.1.6) is an entrance enzyme in the flavonoid biosynthesis, which catalyzes the conversion of chalcones to flavanones. In this study, the full-length CHI cDNA from grape vine (Vitis vinifera L.) was cloned, the recombinant protein was purified and the polyclonal antibody was prepared. Using these tools, the expression and tissue localization of CHI in developing grape berry was analyzed by RT-PCR, gel blot hybridization and immunohistochemical techniques. The expression of CHI was dependent on developmental stage, and CHI protein was mainly distributed in vascular bundles throughout all the stages of berry development, which suggested that flavonoids in the berry might have been partially synthesized in situ.