文献类型：期刊文献

英文题名：Metabolic profiling reveals key metabolites regulating adventitious root formation in ancient Platycladus orientalis cuttings

作者：Chang, Ermei[1,2] Guo, Wei[3] Dong, Yao[4] Jia, Zirui[1] Zhao, Xiulian[1] Jiang, Zeping[4] Zhang, Li[5] Zhang, Jin[2] Liu, Jianfeng[1]

第一作者：Chang, Ermei

通信作者：Liu, JF[1];Zhang, J[2];Zhang, L[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing, Peoples R China;[2]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China;[3]Taishan Acad Forestry Sci, Tai An, Shandong, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forest Ecol, Key Lab Forest Ecol Natl Forestry & Grassland Adm, Beijing, Peoples R China;[5]Heze Univ, Coll Agr & Biol Engn, Heze, Shandong, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001035181600001)】；

基金：This work was supported by the National Non-Profit Research Institutions of the Chinese Academy of Forestry (RIF2014-11 and CAFYBB2020ZD002-4), the State Natural Science Fund projects (31870664), and the open fund of the State Key Laboratory of Subtropical Silviculture (SKLSS-KF2022-09).

语种：英文

外文关键词：ancient tree; lignification; inhibitors; adventitious root formation; wounding

摘要：Platycladus orientalis, a common horticultural tree species, has an extremely long life span and forms a graceful canopy. Its branches, leaves, and cones have been used in traditional Chinese medicine. However, difficulty in rooting is the main limiting factor for the conservation of germplasm resources. This study shows that the rooting rates and root numbers of cuttings were significantly reduced in ancient P. orientalis donors compared to 5-year-old P. orientalis donors. The contents of differentially accumulated metabolites (DAMs) in phenylpropanoid (caffeic acid and coniferyl alcohol) and flavonoid biosynthesis (cinnamoyl-CoA and isoliquiritigenin) pathways increased significantly in cuttings propagated from ancient P. orientalis donors compared to 5-year-old P. orientalis donors during adventitious root (AR) formation. These DAMs may prevent the ancient P. orientalis cuttings from rooting, and gradual lignification of callus was one of the main reasons for the failed rooting of ancient P. orientalis cuttings. The rooting rates of ancient P. orientalis cuttings were improved by wounding the callus to identify wounding-induced rooting-promoting metabolites. After wounding, the contents of DAMs in zeatin (5 & PRIME;-methylthioadenosine, cis-zeatin-O-glucoside, and adenine) and aminoacyl-tRNA biosynthesis (l-glutamine, l-histidine, l-isoleucine, l-leucine, and l-arginine) pathways increased, which might promote cell division and provided energy for the rooting process. The findings of our study suggest that breaking down the lignification of callus via wounding can eventually improve the rooting rates of ancient P. orientalis cuttings, which provides a new solution for cuttings of other difficult-to-root horticultural and woody plants.