文献类型：期刊文献

英文题名：Global poplar root and leaf transcriptomes reveal links between growth and stress responses under nitrogen starvation and excess

作者：Luo, Jie[1,2] Zhou, Jing[1] Li, Hong[3] Shi, Wenguang[1] Polle, Andrea[4] Lu, Mengzhu[1] Sun, Xiaomei[1] Luo, Zhi-Bin[1,2]

第一作者：Luo, Jie

通信作者：Luo, ZB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China;[3]Northwest A&F Univ, Coll Plant Protect, Key Lab Appl Entomol, Yangling 712100, Shaanxi, Peoples R China;[4]Univ Gottingen, Dept Forest Bot & Tree Physiol, Busgen Inst, D-37077 Gottingen, Germany

年份：2015

卷号：35

期号：12

起止页码：1283-1302

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-84948828518);WOS:【SCI-EXPANDED(收录号:WOS:000368440100002)】；

基金：This work was financially supported by the Special Fund on Essential Research for National Non-profit Institutions to Chinese Academy of Forestry (grant no. CAFYBB2014ZX001-2), the State Key Basic Research Development Program (grant no. 2012CB416902), the National Natural Science Foundation of China (grant no. 31100481, 31270647, 31470618) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant no. 20130204110012). Research conducted in the laboratory of A.P. was supported by the Bundesministerium fur Forschung und Technology (BMBF).

语种：英文

外文关键词：nutrient physiology; phytohormone; Populus; RNA sequencing; woody plants

摘要：Nitrogen (N) starvation and excess have distinct effects on N uptake and metabolism in poplars, but the global transcriptomic changes underlying morphological and physiological acclimation to altered N availability are unknown. We found that N starvation stimulated the fine root length and surface area by 54 and 49%, respectively, decreased the net photosynthetic rate by 15% and reduced the concentrations of NH4+, NO3- and total free amino acids in the roots and leaves of Populus simonii Carr. in comparison with normal N supply, whereas N excess had the opposite effect in most cases. Global transcriptome analysis of roots and leaves elucidated the specific molecular responses to N starvation and excess. Under N starvation and excess, gene ontology (GO) terms related to ion transport and response to auxin stimulus were enriched in roots, whereas the GO term for response to abscisic acid stimulus was overrepresented in leaves. Common GO terms for all N treatments in roots and leaves were related to development, N metabolism, response to stress and hormone stimulus. Approximately 30-40% of the differentially expressed genes formed a transcriptomic regulatory network under each condition. These results suggest that global transcriptomic reprogramming plays a key role in the morphological and physiological acclimation of poplar roots and leaves to N starvation and excess.