文献类型：期刊文献

英文题名：Integration of small RNA, degradome, and transcriptome sequencing data illustrates the mechanism of low phosphorus adaptation in Camellia oleifera

作者：Chen, Juanjuan[1,2,3] Han, Xiaojiao[1,2] Ye, Sicheng[1,2] Liu, Linxiu[1,2] Yang, Bingbing[1,2] Cao, Yongqing[1,2] Zhuo, Renying[1,2] Yao, Xiaohua[1,2]

第一作者：Chen, Juanjuan

通信作者：Zhuo, RY[1];Yao, XH[1];Zhuo, RY[2];Yao, XH[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Hangzhou, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Peoples R China;[3]Nanjing Forestry Univ, Forestry Fac, Nanjing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85136233560);WOS:【SCI-EXPANDED(收录号:WOS:000840766400001)】；

基金：Funding This study was supported by the National Key R&D Program of China (2019YFD1001602).

语种：英文

外文关键词：Camellia oleifera; low phosphorus stress; integration analysis; miRNA; co-expression network

摘要：Phosphorus (P) is an indispensable macronutrient for plant growth and development, and it is involved in various cellular biological activities in plants. Camellia oleifera is a unique high-quality woody oil plant that grows in the hills and mountains of southern China. However, the available P content is deficient in southern woodland soil. Until now, few studies focused on the regulatory functions of microRNAs (miRNAs) and their target genes under low inorganic phosphate (Pi) stress. In this study, we integrated small RNA, degradome, and transcriptome sequencing data to investigate the mechanism of low Pi adaptation in C. oleifera. We identified 40,689 unigenes and 386 miRNAs by the deep sequencing technology and divided the miRNAs into four different groups. We found 32 miRNAs which were differentially expressed under low Pi treatment. A total of 414 target genes of 108 miRNAs were verified by degradome sequencing. Gene ontology (GO) functional analysis of target genes found that they were related to the signal response to the stimulus and transporter activity, indicating that they may respond to low Pi stress. The integrated analysis revealed that 31 miRNA-target pairs had negatively correlated expression patterns. A co-expression regulatory network was established based on the profiles of differentially expressed genes. In total, three hub genes (ARF22, WRKY53, and SCL6), which were the targets of differentially expressed miRNAs, were discovered. Our results showed that integrated analyses of the small RNA, degradome, and transcriptome sequencing data provided a valuable basis for investigating low Pi in C. oleifera and offer new perspectives on the mechanism of low Pi tolerance in woody oil plants.